Protein Info for Dshi_0139 in Dinoroseobacter shibae DFL-12

Annotation: type I secretion system ATPase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 transmembrane" amino acids 26 to 48 (23 residues), see Phobius details amino acids 64 to 83 (20 residues), see Phobius details amino acids 137 to 157 (21 residues), see Phobius details amino acids 163 to 183 (21 residues), see Phobius details amino acids 253 to 270 (18 residues), see Phobius details amino acids 276 to 302 (27 residues), see Phobius details TIGR01842: type I secretion system ATPase" amino acids 27 to 562 (536 residues), 662.1 bits, see alignment E=3.2e-203 PF00664: ABC_membrane" amino acids 30 to 286 (257 residues), 64.4 bits, see alignment E=1.4e-21 PF00005: ABC_tran" amino acids 353 to 501 (149 residues), 98.6 bits, see alignment E=4.9e-32

Best Hits

Swiss-Prot: 45% identical to APRD_PSEAE: Alkaline protease secretion ATP-binding protein AprD (aprD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06148, ATP-binding cassette, subfamily C, bacterial (inferred from 100% identity to dsh:Dshi_0139)

Predicted SEED Role

"Type I secretion system ATPase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LKP5 at UniProt or InterPro

Protein Sequence (579 amino acids)

>Dshi_0139 type I secretion system ATPase (RefSeq) (Dinoroseobacter shibae DFL-12)
MAPRTSPNAGKGLEELGAIRRANMPLFAWVFVFSIFVNALMLTGPLYMLQVYDRVLGSQS
EETLLALSVLVAFLFLMMGILDWARGRVLARAGARMQDTLDQRVFSASLRKSLRQGQTTA
STGLRDLESMQRLLSSPALLAVFDIPWTPLFVAAIFMFHPWLGYLALVGGTFLILVTVLN
QWLTSRPVSKANEASARAEMMSQQLAGGAETVESLGMRGAAFTRWSKVRAESLSETLTSS
DRTGGFSTLTKTFRLFLQSAMLGLGAYLVLQGQLTAGAMIAGSILMGRALAPIEMAIGQW
PLVQRAMKGRRDLIELLDAIPPDPERTPLPKPKARLVVEQLIVVPPGEAQAALRSVSFTV
SPGEACGVIGQSGAGKSTLARALTGIWPVAGGKIRLDGAALDQFGPDVLGTHIGYLPQQV
VLFDGTIAENIARLAPQPDAQAVVDAAKRADAHEMILKLPKGYDTMLLGSQTRLSGGQMQ
RIGLARALYGDPVLLVLDEPNSNLDNSGSQALNAAVARMKKDGKAVLIMAHRPAAIEQCD
TLLVLEGGSRKAFGPRDEVLKSMVSNHKEILKKAAGGVS