Protein Info for BT4738 in Bacteroides thetaiotaomicron VPI-5482

Annotation: formate acetyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01255: formate acetyltransferase
  • amino acids 7 to 742 (736 residues), 1100.3 bits, E=0
• PF02901: PFL-like
  • amino acids 15 to 601 (587 residues), 643.5 bits, E=4.8e-197
• PF01228: Gly_radical
  • amino acids 617 to 723 (107 residues), 134.8 bits, E=1.6e-43

Best Hits

Swiss-Prot: 61% identical to PFL_CLOPA: Formate acetyltransferase (pfl) from Clostridium pasteurianum

KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 100% identity to bth:BT_4738)

MetaCyc: 61% identical to pyruvate formate-lyase subunit (Clostridium pasteurianum)
Formate C-acetyltransferase. [EC: 2.3.1.54]

Predicted SEED Role

"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)

MetaCyc Pathways

• pyruvate fermentation to acetate IV (3/3 steps found)
• L-threonine degradation I (5/6 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of N-acetylneuraminate degradation (16/22 steps found)
• superpathway of L-threonine metabolism (13/18 steps found)
• pyruvate fermentation to ethanol I (2/3 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.54

Use Curated BLAST to search for 2.3.1.54

Sequence Analysis Tools

See Q89YJ3 at UniProt or InterPro

Protein Sequence (742 amino acids)

>BT4738 formate acetyltransferase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKAVWNRCLEALAEEREN
NGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGV
EVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALY
GIDRLIEAKKEDLHNLTGPMTDARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKL
RMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLT
VLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFF
GARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLIEIARVYNE
AMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIG
LTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMY
GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPK
SLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
NFVKLSREHQLEVISRSFHERM