Protein Info for BT4684 in Bacteroides thetaiotaomicron VPI-5482

Annotation: beta-galactosidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 895 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 82 to 198 (117 residues), 51.4 bits, see alignment E=2.5e-17 PF00703: Glyco_hydro_2" amino acids 224 to 325 (102 residues), 67.2 bits, see alignment E=4.1e-22 PF02836: Glyco_hydro_2_C" amino acids 333 to 458 (126 residues), 71.4 bits, see alignment E=1.5e-23 PF18565: Glyco_hydro2_C5" amino acids 736 to 815 (80 residues), 50.6 bits, see alignment E=3e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4684)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89YP7 at UniProt or InterPro

Protein Sequence (895 amino acids)

>BT4684 beta-galactosidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKSTSTWWKILLSGLLLAGTLVANADTYQPSYSTAGFYQLSNTGRTVYNMNPAWRFYKGN
IEGAEQPRFNDKDWNIVSLPDGIEYLPTEASGCINYQGEIWYRKHFTPKESWKGKQQFLH
FEAIMGKSKIWVNGKLLKEHFGGFLPVIVDVTSSLKYGEDNVITVWADNSDDPSYPPGKP
QDALDFAYFGGIYRDCWMIVHNKVFITDPNYENETAGGGLFVSFDKVSEQSAQLKLDAHI
RNTSGKSFSGKIIYELYDRDDKRVLSKETGLSVSKDLAKQISCKVTVETPHLWSPDSPYL
YKLHIYIKDKAGNTIDGYYRRIGIRSIEFKGKDGFWLNGKPYPYPLMGANRHQDFAVIGN
ALPNSLHWRDAKKLRDAGMRVIRNAHYPQDPAFMDACDELGLFVIVNTPGWQFWNDQPIF
AQRVYSDIRNMVRRDRNHPSVWMWEPILNETWYPADFAKNVVDILNEEYPYPYCYAGCDV
TARGHEYFPIHFTHPMNGGGGAFNTENLDPKISYFTREWGDNVDDWNSHNSPSRVNRGWG
EVPMLIQAQGYAKNDYQLTSYDGLYRTSRQHMGGCLWHSFDHQRGYHPDPFYGGIMDAFR
QPKLSYYMFCSQRPAEPNKELIADNGPMIYIANAMTPFSPKDVTIYSNCDEVRLTYCKGG
KEYTYHKPANESGMPSPVITFKDVFDVMYDKKLSRQKKQADSYLLAEGLMDGKVVATHKV
TPTRRPSKLLLWADDEKVQMKADGSDIVTVIAAIADENGNIKRLNNYEVKFEIEGQGQLV
ANEETFTNPRPVLWGTAPVLVRSTTTPGKIKIRASVVWQGKHTPVPAELIIPTFPSEHTL
VADKDELTQAQSASKDAGNKINAAPSDCEKRVLELQQELNRLKLKEVEKQQSDFE