Protein Info for BT4667 in Bacteroides thetaiotaomicron VPI-5482

Annotation: beta-galactosidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 815 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 30 to 191 (162 residues), 57.5 bits, see alignment E=5.9e-19 PF00703: Glyco_hydro_2" amino acids 206 to 304 (99 residues), 68.3 bits, see alignment E=3.5e-22 PF02836: Glyco_hydro_2_C" amino acids 309 to 536 (228 residues), 88.9 bits, see alignment E=1.2e-28 PF16355: DUF4982" amino acids 632 to 696 (65 residues), 83.5 bits, see alignment E=2.8e-27 PF18565: Glyco_hydro2_C5" amino acids 709 to 810 (102 residues), 109.8 bits, see alignment E=1.9e-35

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 100% identity to bth:BT_4667)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89YR4 at UniProt or InterPro

Protein Sequence (815 amino acids)

>BT4667 beta-galactosidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNMKHQKQLFTVLLMSAACMLQAQRSETLLEKNWKFSKGDFPEAAQTDYNDTKWESVVIP
HDWAIFGPFDMNNDLQNVAVTQNFEKKASLKTGRTGGLPYVGTGWYRTSFDVPADKEVTL
LFDGAMSEARVYVNGQEACFWPFGYNSFHCNVTPFLNKDGKNNTLAVRLENRPQSSRWYP
GAGLYRNVHLIVTEKVHIPVWGTQITTPHVSDEFAAVRLQTKIENAGEKTEIRIETEILS
PDGKVVTRKENKGRINHGQPFEQNFIVNAPQLWSPESPALYKAVSKVYADNQLVDTYSTR
FGIRSIEYIADKGFYLNGKHRKFQGVCNHHDLGPLGAAINVAALRHQLTLLKDMGCDAIR
TSHNMPAPELVELCDEMGFMMMIEPFDEWDIAKCENGYHRYFNEWAERDMVNMLHNYRNN
PCVVMWSIGNEVPTQCSPVGYKVAKFLQDICHREDPTRPVTCGMDQVTCVLANGFAAMID
IPGLNYRANRYQEAYDKLPQNLILGSETASTVSSRGVYKFPVKDKKSAQYDDHQCSSYDV
EACSWSNIPDEDFALADDHHWTIGQFVWTGFDYLGEPSPYDTDAWPNHSSMFGIIDLASL
PKDRYYLYRSIWNKEAETLHILPHWTWPGREGEVTPVFVYTNYPAAELFINGKSYGKQSK
NNSSLKSRYRLMWMDAIYEPGEVKVVAYDKDGKAVAEKSVRTASKPHHIELVSNRNELTA
DGKDLAYVTVKVVDKDGNLCPADSRLINFSVKGAGKYRAGANGDPTSLDLFHLPKMHAFN
GMLTAIVQTNETAGEIILTAKAGGVKAGSIRLLTK