Protein Info for BT4554 in Bacteroides thetaiotaomicron VPI-5482

Annotation: purine nucleoside phosphorylase II (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01048: PNP_UDP_1
  • amino acids 32 to 269 (238 residues), 133.4 bits, E=4.5e-43

Best Hits

KEGG orthology group: K00757, uridine phosphorylase [EC: 2.4.2.3] (inferred from 100% identity to bth:BT_4554)

Predicted SEED Role

"Purine nucleoside phosphorylase (EC 2.4.2.1)" in subsystem Adenosyl nucleosidases or Deoxyribose and Deoxynucleoside Catabolism or Purine conversions (EC 2.4.2.1)

MetaCyc Pathways

• superpathway of purine nucleotide salvage (13/14 steps found)
• adenine and adenosine salvage I (2/2 steps found)
• guanine and guanosine salvage I (2/2 steps found)
• pyrimidine ribonucleosides degradation (2/2 steps found)
• xanthine and xanthosine salvage (2/2 steps found)
• adenine and adenosine salvage III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine deoxyribonucleosides degradation I (3/4 steps found)
• purine deoxyribonucleosides degradation II (2/3 steps found)
• pyrimidine deoxyribonucleosides degradation (2/3 steps found)
• superpathway of guanine and guanosine salvage (2/3 steps found)
• purine ribonucleosides degradation (4/6 steps found)
• guanosine nucleotides degradation III (2/4 steps found)
• purine nucleotides degradation II (aerobic) (7/11 steps found)
• superpathway of purine deoxyribonucleosides degradation (4/7 steps found)
• adenine and adenosine salvage V (1/3 steps found)
• nucleoside and nucleotide degradation (halobacteria) (3/6 steps found)
• superpathway of pyrimidine deoxyribonucleosides degradation (3/6 steps found)
• adenosine nucleotides degradation II (2/5 steps found)
• superpathway of pyrimidine ribonucleosides degradation (2/5 steps found)
• ureide biosynthesis (3/7 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)
• fluoroacetate and fluorothreonine biosynthesis (1/6 steps found)
• arsenic detoxification (mammals) (7/17 steps found)
• salinosporamide A biosynthesis (4/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.1

Use Curated BLAST to search for 2.4.2.1 or 2.4.2.3

Sequence Analysis Tools

See Q89Z23 at UniProt or InterPro

Protein Sequence (292 amino acids)

>BT4554 purine nucleoside phosphorylase II (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKKYFPSSELIINEDGSVFHLHVKPEWLADKVILVGDPGRVALVASHFENKECEVESREF
KTITGTYKGKRITVVSTGIGCDNIDIVMNELDALANINFETREEKEKFRQLELVRIGTCG
GLQPNTPVGTFVCSQKSIGFDGLLNFYAGRNAVCDLPFERAFLNHMGWSGNMCAPAPYVI
DASEELIDRIAKEDMVRGVTIAAGGFFGPQGRELRIPLADPKQNDKIEAFEYKGFKITNF
EMESSALAGLSRLMGHKAMTVCMVIANRLIKEANTGYKNTIDTLIKTVLDRI