Protein Info for BT4535 in Bacteroides thetaiotaomicron VPI-5482

Annotation: Type I restriction enzyme EcoR124II R protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 942 PF04313: HSDR_N" amino acids 4 to 188 (185 residues), 124.6 bits, see alignment E=9.7e-40 TIGR00348: type I site-specific deoxyribonuclease, HsdR family" amino acids 9 to 656 (648 residues), 457.1 bits, see alignment E=5.7e-141 PF04851: ResIII" amino acids 241 to 404 (164 residues), 134.5 bits, see alignment E=7.1e-43 PF18766: SWI2_SNF2" amino acids 246 to 464 (219 residues), 204.2 bits, see alignment E=4.5e-64 PF12008: EcoR124_C" amino acids 662 to 939 (278 residues), 191.1 bits, see alignment E=5.9e-60

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 100% identity to bth:BT_4535)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89Z42 at UniProt or InterPro

Protein Sequence (942 amino acids)

>BT4535 Type I restriction enzyme EcoR124II R protein (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MSTQSEAALEAGLIATLRQMDYEYVQIVEEDNLYANFKRQLEIHNRKRLEEHGRTEFTAE
EFEKILIYLEGGTRFEKAKKLRDLYPLDTADGQRIWVEFLNRQQWCQNEFQVSNQITVEG
RKKCRYDVTILINGLPLVQIELKRRGVELKQAYNQIQRYHKTSFHGLFDYIQLFVISNGV
NTRYFANNPNSGYKFTFNWTDAANHPFNELDKFAVFFLEKCTLGKIIGKYIVLHEGDKCL
MVLRPYQFYAVEKILDRVQNSNDNGYIWHTTGAGKTLTSFKAAQLVSELDDVDKVMFVVD
RHDLDTQTQSEYEAFEPGAVDSTDNTDELVKRLQSNSKIIITTIQKLNAAVSKTWYSSKI
DAIRHSRIVMIFDECHRSHFGDSHKKIMKFFGNAQIFGFTGTPIFTENAVDGHTTKEIFG
NCLHKYLIKDAIADENVLGFLVEYYHGNEIVDNDNQARMEEIAKFILNNFNKSTFDGEFD
ALFAVQSVPMLIRYYKIFRSLNPKIRIGAVFTYAANNSQDDEQTGMGTGQYAKESVGEAD
ELQAIMDDYNENFGTSFTTENFRAYYDDINLRMKKKKADMKPLDLCLVVGMFLTGFDSKK
LNTLYVDKNMEYHGLLQAFSRTNRVLNEKKRFGKIVCFRDLKNNVDTSIKLFSNSDNPED
IVRPPFEKVKQEYKSLSTDFLQKYPTPSSIDFLQSENDKKNFVLAFRDIIRKHAEIQIYE
DYSEDAEDLGMTEQQFNDYKSKYLDITVGFIDPPAAPSTVAEDPVPYGNNQGLEDIDFCL
ELLHSDIINVAYILALINDLDPYSDDYSEKRQNIIDTMIKDAGMRGKAKLIDGFIKKNVD
EDKENFMNGRNKADGTNELEERLNQYIVSERNKAVNDLADEEQISAEVLNHYMKEYDYLQ
KEQPEIIQKALKEKHLGLIKTRRALTRIMERLRSIIKTFNWE