Protein Info for BT4523 in Bacteroides thetaiotaomicron VPI-5482

Annotation: Type I restriction enzyme EcoR124II specificity protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 394 PF01420: Methylase_S" amino acids 13 to 183 (171 residues), 48.6 bits, see alignment E=5.8e-17

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4523)

Predicted SEED Role

"Type I restriction-modification system, specificity subunit S (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89Z54 at UniProt or InterPro

Protein Sequence (394 amino acids)

>BT4523 Type I restriction enzyme EcoR124II specificity protein (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MPHYENVPFEIPNSWVWTTIEEICSKIGSGSTPRGSNYSANGIPFFRSQNVYNDRLVYDD
IKYISEEVHQKMKGTEVLANDLLLNITGGSLGRCAVVPADFNCGNVSQHVCIMRSVLVEP
EYFHALVLSSYFAKSMKITGSGREGLPKYSLEQMAFPLPPLSEQQRIVMEIEKLFALIDQ
IEHSKVNLQTIIKQTKSKILDLAIHGKLVPQDPNDEPAIELLKRINPDFTPCDNGHYTQL
PDGWTFCRLDQIIGYEQSTAYIVESTAYDDSYSTPVLTAGKSFIIGYTNEATGIYSNLPC
IIFDDFTTDSKLVDFPFKVKSSAMKILKVHKDIEVDYVAMFMSITKLVGDTHKRYWISEY
SKLEIPIPSKAEQKRIIHAIHGIFTQLDLIMESL