Protein Info for BT4456 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative electron transport protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 PF02589: LUD_dom" amino acids 65 to 283 (219 residues), 144.5 bits, see alignment E=1.4e-45 PF13183: Fer4_8" amino acids 301 to 366 (66 residues), 50.1 bits, see alignment E=1.3e-16 PF13534: Fer4_17" amino acids 302 to 367 (66 residues), 31.9 bits, see alignment E=6.4e-11 PF12800: Fer4_4" amino acids 302 to 316 (15 residues), 19.1 bits, see alignment (E = 5.2e-07) amino acids 350 to 364 (15 residues), 11.5 bits, see alignment (E = 0.00015) PF12838: Fer4_7" amino acids 302 to 366 (65 residues), 31.8 bits, see alignment E=6.6e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4456)

MetaCyc: 53% identical to L-lactate dehydrogenase iron-sulfur cluster-binding protein LldF (Shewanella oneidensis)
L-lactate dehydrogenase (cytochrome). [EC: 1.1.2.3]

Predicted SEED Role

"Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF" in subsystem L-rhamnose utilization or Lactate utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89ZC1 at UniProt or InterPro

Protein Sequence (461 amino acids)

>BT4456 putative electron transport protein (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MSTKHSKAAEKFLQDSKMAAWHNETLWMVRAKRDKMSKEVPEWEELRNKACELKLYSNSH
LEELLQEFEKNATANGAIVHWAKDADEYCAIVYEILNEHNVHHFIKSKSMLAEECGLNPF
LMERGIDVVESDLGERILQLMHLEPSHIVLPAIHIKREQVGELFEKEMGTEKGNFDPTYL
THAARKNLRHLFLNAEAAMTGANFAVASTGDIVVCTNEGNADMGTSYPKLNIAAFGMEKI
VPDLDALGVFTRLLARSATGQPVTTYTSHYRRPREGGEYHIIIVDNGRSTILSKPDHIKT
LNCIRCGACMNTCPVYRRSGGYSYTYFIPGPIGINLGMAHDPEKYYDNLSACSLCMSCSD
VCPVKVDLAEQIYKWRQDLDGLGKANTGKKIMSGGMKFLMERPALFNAALWAAPMVNGLP
RFMKYNDFDDWGKGRELPEFAKESFNEMWKKNEVQGKEESK