Protein Info for BT4417 in Bacteroides thetaiotaomicron VPI-5482

Annotation: HD superfamily hydrolase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 511 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF12072: RNase_Y_N" amino acids 4 to 195 (192 residues), 116.5 bits, see alignment E=2.3e-37 TIGR03319: ribonuclease Y" amino acids 8 to 511 (504 residues), 640.9 bits, see alignment E=1.3e-196 PF00013: KH_1" amino acids 210 to 259 (50 residues), 29.1 bits, see alignment 1.4e-10 TIGR00277: HDIG domain" amino acids 323 to 399 (77 residues), 64.3 bits, see alignment E=7.6e-22 PF01966: HD" amino acids 328 to 418 (91 residues), 68.9 bits, see alignment E=9.3e-23

Best Hits

Swiss-Prot: 100% identical to RNY_BACTN: Ribonuclease Y (rny) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K06950, uncharacterized protein (inferred from 100% identity to bth:BT_4417)

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.16

Use Curated BLAST to search for 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q89ZG0 at UniProt or InterPro

Protein Sequence (511 amino acids)

>BT4417 HD superfamily hydrolase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MIAIIATAIACFVVGGILSYVLFRYVLKSKYDSVLKDAETEAEVIKKNKLLEVKEKFLNK
KADLEKEVALRNQKIQQAENKLKQREMVLSQRQEEIQRKKLEAEAVKENLEAQLVIVDKK
KEELDKLQHQEIEKLEAISGLSADEAKERLVESLKEEAKTQAQSFINDIMDDAKLTASKE
AKRIVIQSIQRVATETAIENSVTVFHIESDEIKGRIIGREGRNIRALEAATGVEIVVDDT
PEAIVLSAFDPVRREIARLALHQLVTDGRIHPARIEEVVSKVRKQVEEEIIETGKRTTID
LGIHGLHPELIRIIGKMKYRSSYGQNLLQHARETANLCAVMASELGLNPKKAKRAGLLHD
IGKVPDEEPELPHALLGMKLAEKYKEKPDICNAIGAHHDETEMTSLLAPIVQVCDAISGA
RPGARREIVEAYIKRLNDLEQLAMAYPGVTKTYAIQAGRELRVIVGADKIDDKQTESLSG
EIAKKIQDEMTYPGQVKITVIRETRAVSFAK