Protein Info for BT4187 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): exo-alpha-D-galacturonosidase for catabolism of rhamnogalacturonan I and polygalacturonate
Rationale: Specifically important in carbon source Rhamnogalacturonan - from potato; carbon source polygalacturonic acid. Rhamnogalacturonan I is a polymer of D-galacturonate and L-rhamnose. This protein is 64% identical to BT4123, which is an exo-alpha-D-galacturonidase (PMC:PMC5784806). BT4187 is expected to be secreted, but no specific signal for protein export was identified by PSORT-b.
Original annotation: exo-poly-alpha-D-galacturonosidase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 538 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF12708: Pectate_lyase_3" amino acids 59 to 114 (56 residues), 32.2 bits, see alignment 9.8e-12 PF00295: Glyco_hydro_28" amino acids 239 to 412 (174 residues), 104.5 bits, see alignment E=6.3e-34

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4187)

Predicted SEED Role

"Polygalacturonase (EC 3.2.1.15)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.2.1.15)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.15

Use Curated BLAST to search for 3.2.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A034 at UniProt or InterPro

Protein Sequence (538 amino acids)

>BT4187 exo-alpha-D-galacturonosidase for catabolism of rhamnogalacturonan I and polygalacturonate (Bacteroides thetaiotaomicron VPI-5482)
MNTMTRRLLWMVVCCLPFVSGCKQSECAISENAIDDTIYQNLPFEMPKVQQPVFPAYEVN
ISKFGAKGDGMTLNTKAINDAIKEVNQRGGGKVIIPEGTWLTGPIELLSNVNLYTERNAL
ILFTGDFEAYPIIPTSFEGLETRRCQSPISARNAENIAITGYGIFDGNGDCWRPVKKEKL
TASQWNKLVKSGGVLDEQERIWYPTAGSLKGAMACKDFNVPEGINTDEEWNEIRAWLRPV
LLNFVKSKRILLEGVTFKNSPSWCLHPLSCEDFTVNNIQVINPWYSQNGDALDLESCKNA
LILNSVFDAGDDAICIKSGKDENGRRRGEPCQNVIVKNNTVLHGHGGFVVGSEMSGGVKN
IYVEDCTFLGTDVGLRFKSTRGRGGVVENIYINNINMINIPNEPLLFDLFYGGKGAGEES
EEDLLSRMKTAIPPVTEETPAFRDIHISNVICRGSGRAMFFNGLPEMPIRNVTVKNVVMT
EATDGVVISQVDGVTLENIYVESTKGKNILNVKSAKNLKVDGETYEEIDAKGQILNFK