Protein Info for BT4160 in Bacteroides thetaiotaomicron VPI-5482

Annotation: beta-galactosidase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01301: Glyco_hydro_35
  • amino acids 44 to 391 (348 residues), 174.5 bits, E=4.2e-55
• PF02449: Glyco_hydro_42
  • amino acids 62 to 145 (84 residues), 28.5 bits, E=1e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4160)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

• lactose degradation III (1/1 steps found)
• xyloglucan degradation II (exoglucanase) (5/8 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Glycosaminoglycan degradation
• Glycosphingolipid biosynthesis - ganglio series
• Other glycan degradation
• Sphingolipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

See Q8A061 at UniProt or InterPro

Protein Sequence (698 amino acids)

>BT4160 beta-galactosidase precursor (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKKKITFLLMFVLSLSMVSAQGTFRFGTSATPEGKTLLVDSKGLILDGKHIIPVMGEIHY
SRVPESEWRREIRKMKAGGINIISTYIFWIHHEPEEGKWNWSGNHNLRRFVRICAEENVM
LVLRLGPFCHGEVYQGGIPSWVHEKAGQNPKYKIRARTPGFLEDCTELYNTIFAQVNGLL
WKDGGPVVGVQIENESRGPWDYLEALKNIAVKAGFDVPFYTRTGWPALRGKEVFGQLLPL
YGDYADGFWDRKLEDMPGSYADAFIMRDKRMSSAIATETFSKEELSEDSPSLSSKLSYPY
FTCELGGGMMPAYHRRININGKELKPLVICKLGSGSNLPGYYMYHGGTNPYNPLHTMGET
QSSPGTNHNDLPHMTYDFQAPLGEVGQVFETPFHEGRFIHQMLTDWGSELLQMNVDSLSR
HYARRGAFEFYNDYVRIKNESGTSHVTFKDYRTEGATIDWTTVEPFCKVDGLIYFIEIRG
KKPQISVDGKVYTCKLNKQQKAGKLNVCVLSYEKAKTAYKIDGKLLYAKNGGILYKSDSC
IVEEVWTKSPVIAATVTEVKKADAPRVVPMGRQAVAAQPVEEDFAKAAVYTINYDTSGIN
NYDNLFLRINYRGDVARVYADGRLVADNFWNGKEMWVRMADLVGKKVELKILPLRKDAPV
YFQKEQKAMIEATKGDYMLGLDSVEVIERHTLEFNDTF