Protein Info for BT4072 in Bacteroides thetaiotaomicron VPI-5482

Annotation: alpha-mannosidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 849 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01074: Glyco_hydro_38N" amino acids 12 to 195 (184 residues), 49.5 bits, see alignment E=6e-17 PF07748: Glyco_hydro_38C" amino acids 488 to 691 (204 residues), 40.8 bits, see alignment E=4.7e-14 PF17677: Glyco_hydro38C2" amino acids 780 to 836 (57 residues), 22.1 bits, see alignment 2e-08

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4072)

Predicted SEED Role

"Alpha-mannosidase (EC 3.2.1.24)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Mannose Metabolism (EC 3.2.1.24)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.24

Use Curated BLAST to search for 3.2.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A0E9 at UniProt or InterPro

Protein Sequence (849 amino acids)

>BT4072 alpha-mannosidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNRLNNIILLVVFICTHAHSQQAYFVDGYHGGIYGHYPVKWKTQFIVDQLSKHPDWRICL
EIEPETWDTVQIQTPGAYRQLKNVVVTKQVEFTNPTYAQPYCYNISGESIIRQFQYGIAK
INKHFPGVTFTTYSVEEPCFTSCLPQILKLFGFKYAVLKCPNTCWGGYTNAYGGELVNWV
GPDGTPILTVPRYACEKLEENSTWQTTAWCNEESYLNACRDAGIEHPVGMCFQDAGWKNG
PWLGSGKNTKSNSVYVTWKDYFENISIGKTNDDWHFSQEDIRVNLMWGSQVLQRIAQEVR
TSENKIIIAEKMSVIAHLANGYTCVQEDLDEAWRTLMLAQHHDSWIVPYNGLNKFGTWAD
QIKRWTDETNTVADKITAASIFSFDNDTINTEKAQGFVRVYNTLGTKRKELVTVELPQEY
ADFDLEVNDYRNKKVDYSIGKEGGKIRLLFEADVPPFGYSTYRIKQVKTGKRTVSGSEKI
INEGEYVVENDMYKIVFDLSKGGIIKSLVAKKEENKEFAKQSGEYSIGELRGYFYDEGKF
RSSTEAPAKLTVLRSNIQETKVKLEGKIASHPFVQIISIAQGEKRIDFDLTINWKKNVGI
GEYKENSWRDNRRAYCDDRFKLSVLFPVNLHSPRIYKNAPFDVCESKLDDTFFNSWDQIK
HNIILHWVDLAEQEGDYALALFADHTTSYSHGKDYPLGLTAQYSGNGLWGPDYKITGPLK
MKYAIVPHRGKWDKAAIATKSDCWNEPLLCSYHSFAKLESRSLVDLKNTGYQVSAANIKD
GKIILRLFNAEGDRKQHNITFDMPLSSIEEVDLNGRVIDRKTIKTRTGKSEISISMPRFG
LKTFALNLN