Protein Info for BT3995 in Bacteroides thetaiotaomicron VPI-5482

Annotation: alanyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to SYA_BACTN: Alanine--tRNA ligase (alaS) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 100% identity to bth:BT_3995)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

tRNA charging (21/21 steps found)
shinorine biosynthesis (3/8 steps found)
vanchrobactin biosynthesis (2/8 steps found)
superpathway of chorismate metabolism (38/59 steps found)
ergotamine biosynthesis (4/12 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
bacillibactin biosynthesis (3/12 steps found)
enterobactin biosynthesis (2/11 steps found)
superpathway of ergotamine biosynthesis (5/18 steps found)
sulfazecin biosynthesis (1/16 steps found)
phosalacine biosynthesis (3/25 steps found)
phosphinothricin tripeptide biosynthesis (3/25 steps found)
corallopyronin A biosynthesis (2/30 steps found)
colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A0M6 at UniProt or InterPro

Protein Sequence (872 amino acids)

>BT3995 alanyl-tRNA synthetase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MLTAKEIRDSFKNFFESKGHHIVPSAPMVIKDDPTLMFTNAGMNQFKDIILGNHPAKYHR
VADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFKKEAINWAWEYLVEVLKL
NPEHLYATVFEGSPEEGLSRDDEAASYWEQYLPKDHIINGNKHDNFWEMGDTGPCGPCSE
IHIDLRPAEERAKISGRDLVNHDHPQVIEIWNLVFMQYNRKADGSLEPLPAKVIDTGMGF
ERLCMALQGKTSNYDTDVFQPMLKAIAAMSGTEYGKDKQQDIAMRVIADHIRTIAFSITD
GQLPSNAKAGYVIRRILRRAVRYGYTFLGQKQSFMYKLLPVLIDNMGDAYPELIAQKGLI
EKVIKEEEEAFLRTLETGIRLLDKTMGDTKAAGKTEISGKDAFTLYDTFGFPLDLTELIL
RENGMTVNIEEFNAEMQQQKQRARNAAAIETGDWVTLREGTTEFVGYDYTEYEASILRYR
QIKQKNQTLYQIVLDCTPFYAESGGQVGDTGVLVSEFETIEVIDTKKENNLPIHITKKLP
EHPEAPMMACVDTDKRAACAANHSATHLLDSALREVLGEHIEQKGSLVTPDSLRFDFSHF
QKVTDEEIRQVEHLVNAKIRANIPLKEYRNIPIEEAKELGAIALFGEKYGERVRVIQFGS
SIEFCGGIHVAATGNIGMVKIISESSVAAGVRRIEAYTGARVEEMLDTIQDTISELKSLF
NNAPDLGIAIRKYIEENAGLKKQVEDYMKEKEASLKERLLKNIQEIHGIKVIKFCAPLPA
EVVKNIAFQLRGEITENLFFVAGSLDNGKPMLTVMLSDNLVAGGLKAGNLVKEAAKLIQG
GGGGQPHFATAGGKNTDGLNAAIEKVLELAGI