Protein Info for BT3932 in Bacteroides thetaiotaomicron VPI-5482

Annotation: DNA primase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 730 TIGR01391: DNA primase" amino acids 1 to 419 (419 residues), 456.3 bits, see alignment E=4.8e-141 PF01807: zf-CHC2" amino acids 3 to 97 (95 residues), 120.1 bits, see alignment E=9.1e-39 PF08275: DNAG_N" amino acids 123 to 255 (133 residues), 120.9 bits, see alignment E=1.1e-38 PF13155: Toprim_2" amino acids 265 to 349 (85 residues), 75.6 bits, see alignment E=9.9e-25 PF01751: Toprim" amino acids 265 to 329 (65 residues), 38.2 bits, see alignment E=3.9e-13 PF13662: Toprim_4" amino acids 266 to 337 (72 residues), 60.6 bits, see alignment E=4.1e-20 PF10410: DnaB_bind" amino acids 372 to 422 (51 residues), 28.5 bits, see alignment 4.4e-10

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 100% identity to bth:BT_3932)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A0T9 at UniProt or InterPro

Protein Sequence (730 amino acids)

>BT3932 DNA primase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MIDQVTIDRILDAAQIVDVVSEFVTLRKRGVNYVGLCPFHNEKTPSFSVSPAKGLCKCFS
CGKGGNSVHFIMEHEQMSYYEALKYLAKKYNIEIKERELTNEEKQAQTTRESMFIVNNFA
RDYFQNILKNHVDGRSIGLAYFRQRGFRDDIIEKFQLGYCTESHDAMSQEALRKGYKKEF
LVKTGICYETDDHRLRDRFWGRVIFPVHTLSGKVVAFGGRVLSTATKGVKVKYVNSPESE
IYHKSNELYGIYFAKQAIVRQDRCFLVEGYTDVISMHQSGIENVVASSGTALTPGQIRLI
HRFTNNITVLYDGDVAGIKASIRGIDMLLEEGMNIKVCLLPDGDDPDSFARKHNSEEFQA
FIREHEKDFIRFKTDLLMEDAGRDPIKRAELISNIVRSISVIPEAIIRDVYIKECSQHLR
IEEKLLVAEVAKLREAQAEKANRPSYNHSSSTTGDTSGTAAASGTPAYSGAPTYSTPSSP
YANAGMPPEPEYDDDGNIVSFADPMSAATGGAAGFPPGNAPASTTDTTVPGDSYTSFIPQ
EGKEGQEFYKFERLILQAVVRYGEKVMCNLTDEEGNEIPVTVIEYVVNDLKEDDLAFHNP
LHRQILTEAATHIHDAGFIAERYFLAHPDQTISRLSVDLINVRYQLSKYHSKSQKIVTDE
ERLYELVPMLMINFKYAIVTEELKHMLYALQDPALAHDNEKCNSLMQRYNEMREVQSLMA
KRLGDRVVLR