Protein Info for BT3917 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative inorganic polyphosphate/ATP-NAD kinase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 PF01513: NAD_kinase" amino acids 16 to 132 (117 residues), 71.6 bits, see alignment E=8.1e-24 PF20143: NAD_kinase_C" amino acids 156 to 280 (125 residues), 101.6 bits, see alignment E=2.7e-33

Best Hits

Swiss-Prot: 100% identical to NADK_BACTN: NAD kinase (nadK) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00858, NAD+ kinase [EC: 2.7.1.23] (inferred from 100% identity to bth:BT_3917)

Predicted SEED Role

"NAD kinase (EC 2.7.1.23)" in subsystem NAD and NADP cofactor biosynthesis global (EC 2.7.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A0V4 at UniProt or InterPro

Protein Sequence (302 amino acids)

>BT3917 putative inorganic polyphosphate/ATP-NAD kinase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MPILQPKLMKQLMKFAIFGNTYQPKKSLHALRLFELLKKQGAEICMCREFYQFLTADLKM
EVPVDALLEGNDFTADMVISIGGDGTFLKAARRVGRKQIPILGINTGRLGFLADVSPEEM
EVTFEEIQAGRYSVEERSVLQLICNDRNLQESPYALNEIAVLKRDSSSMISIRTAINGAY
LNTYQADGLVIATPTGSTAYSLSVGGPIIVPHSNTIAITPVAPHSLNVRPIVIRDDWEIT
LDVESRSHNFLVAIDGSSETCKETTQLTIRRADYSIKVVKRFNHIFFDTLRSKMMWGADG
RR