Protein Info for BT3755 in Bacteroides thetaiotaomicron VPI-5482

Annotation: acetyl-coenzyme A synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 37 to 409 (373 residues), 218.5 bits, E=1.4e-68
• PF13193: AMP-binding_C
  • amino acids 459 to 539 (81 residues), 85.7 bits, E=3.4e-28

Best Hits

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to bth:BT_3755)

Predicted SEED Role

"Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases"

MetaCyc Pathways

• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation II (2/3 steps found)
• ethanol degradation IV (2/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• ethanol degradation III (1/3 steps found)
• chitin deacetylation (1/4 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (1/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.1

Sequence Analysis Tools

See Q8A1B1 at UniProt or InterPro

Protein Sequence (551 amino acids)

>BT3755 acetyl-coenzyme A synthetase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MVERFLAQTSFASQEDFIKNLKINVPENFNFGYDVVDAWAAEQPDKNALLWTNDQGESRQ
FSFADMKRYTDMTASYFQSLGIGRGDMVMLILKRRYEFWYSTIALHKLGATVIPATHLLT
KKDIIYRCNAADIKMIVAAGEGIILQHIKDALPECPSVEKLVSVGPEVPEGFEDFHQGID
NAAPFIRPRHANTNDDISLMYFTSGTTGEPKMVAHDFTYPLGHIVTGSFWHNLDENSLHL
TIADTGWGKAVWGKLYGQWIAGANIFVYDHEKFTPAAILEKIQEYQVTSLCAPPTIFRFL
IHEDLTKYDLSSLRYCTIAGEALNPAVFETFKKLTGIKLMEGFGQTETTLTVATMPWMEP
KPGSMGLPNPQYDVDLIDSEGRSVEAGEQGQIVIRTSKGKPLGLFKEYYRDAERTHEAWH
DGIYYTGDVAWKDEDGYLWFVGRADDVIKSSGYRIGPFEVESALMTHPAVIECAITGVPD
EIRGQVVKATIVLSKDYKARAGEELIKELQNHVKKVTAPYKYPRVIEFVDELPKTISGKI
RRVEIRENDEK