Protein Info for BT3722 in Bacteroides thetaiotaomicron VPI-5482

Annotation: glutamate racemase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 37% identical to MURI_BACSK: Glutamate racemase (murI) from Bacillus clausii (strain KSM-K16)

KEGG orthology group: K01776, glutamate racemase [EC: 5.1.1.3] (inferred from 100% identity to bth:BT_3722)

Predicted SEED Role

"Glutamate racemase (EC 5.1.1.3)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Peptidoglycan Biosynthesis (EC 5.1.1.3)

MetaCyc Pathways

peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1E4 at UniProt or InterPro

Protein Sequence (280 amino acids)

>BT3722 glutamate racemase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKQHLSHTPGPIGVFDSGYGGLTILNKIREALPEYDYVYLGDNARTPYGTRSFEIVYEFT
LQAVNKLFEMGCHLVILACNTASAKALRSIQMNDLPGIDPERRVLGVIRPTVECIGGITQ
SRHIGVLATAGTIKSESYPLEIHKLFPDIQVSGMACPMWVPLVENNESQNEGADYFIRKY
IDQLLSKDPEIDTMILGCTHYPILLPKIQEYTPKHIRIVAQGEYVAESLKDYLSRHPEMN
AKCTQNGSCLFYTTEAEEKFVESASSFLNQQIDVRRISLE