Protein Info for BT3717 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): N-succinylornithine carbamoyltransferase (EC 2.1.3.11)
Rationale: Important for fitness in most defined media, except when arginine is provided. Over 90% identical to the N-succinylornithine carbamoyltransferase of B. fragilis (E1WKT5)
Original annotation: ornithine carbamoyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 PF02729: OTCace_N" amino acids 12 to 160 (149 residues), 86.1 bits, see alignment E=2.7e-28 PF00185: OTCace" amino acids 184 to 309 (126 residues), 66 bits, see alignment E=4.4e-22

Best Hits

Swiss-Prot: 100% identical to AOTC_BACTN: N-acetylornithine carbamoyltransferase (argF') from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K13043, N-succinyl-L-ornithine transcarbamylase [EC: 2.1.3.11] (inferred from 100% identity to bth:BT_3717)

MetaCyc: 100% identical to N-succinylornithine carbamoyltransferase (Bacteroides thetaiotaomicron)
N-succinylornithine carbamoyltransferase. [EC: 2.1.3.11]

Predicted SEED Role

"N-acetylornithine carbamoyltransferase (EC 2.1.3.9)" in subsystem Arginine Biosynthesis extended (EC 2.1.3.9)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.3.11 or 2.1.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1E9 at UniProt or InterPro

Protein Sequence (318 amino acids)

>BT3717 N-succinylornithine carbamoyltransferase (EC 2.1.3.11) (Bacteroides thetaiotaomicron VPI-5482)
MKKFTCVQDIGDLKSALAEAFEIQKDRFKYVELGRNKTLMMIFFNSSLRTRLSTQKAALN
LGMNVMVLDINQGAWKLETERGVIMDGDKPEHLLEAIPVMGCYCDIIGVRSFARFEDRDF
DYQETILNQFIQYSGRPVFSMEAATRHPLQSFADLITIEEYKKTARPKVVMTWAPHPRPL
PQAVPNSFAEWMNATDYDFVITHPEGYELAPQFVGNAKVEYDQMKAFEGADFIYAKNWAA
YTGDNYGQILSKDREWTVSDRQMAVTNNAFFMHCLPVRRNMIVTDDVIESPQSIVIPEAA
NREISATVVLKRLIEGLE