Protein Info for BT3704 in Bacteroides thetaiotaomicron VPI-5482

Name: SusA
Updated annotation (from data): SusA: alpha-amylase / neopullulanase / cyclomaltodextrinase (EC 3.2.1.135; EC 3.2.1.54)
Rationale: Specifically important in carbon source a-Cyclodextrin SusA. Some homlogs are also specifically required for utilizing cyclodextrin. SusA was previously shown to account for most of the neopullulanase activity of cell extracts (PMCID:PMC178630)
Original annotation: alpha-amylase (neopullulanase) SusA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF09087: Cyc-maltodext_N" amino acids 28 to 113 (86 residues), 106.6 bits, see alignment E=1.4e-34 PF00128: Alpha-amylase" amino acids 165 to 482 (318 residues), 194.3 bits, see alignment E=8.6e-61 PF10438: Cyc-maltodext_C" amino acids 542 to 615 (74 residues), 86.8 bits, see alignment E=1.7e-28

Best Hits

Swiss-Prot: 100% identical to SUSA_BACTN: Neopullulanase SusA (susA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: None (inferred from 100% identity to bth:BT_3704)

Predicted SEED Role

"Alpha-amylase (Neopullulanase) SusA (EC 3.2.1.135)" (EC 3.2.1.135)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.135 or 3.2.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1G0 at UniProt or InterPro

Protein Sequence (617 amino acids)

>BT3704 SusA: alpha-amylase / neopullulanase / cyclomaltodextrinase (EC 3.2.1.135; EC 3.2.1.54) (Bacteroides thetaiotaomicron VPI-5482)
MKRNLLFIILLLLLPGLHQVFATSTIKKVAPTFWWAGMKNPELQILLYGDRISSADVSLS
ADNITLQEVVKQENPNYLVLYLDLSKAAPQNFDIILKQGKKQTKIPYELKQRRPNASAVE
GFDSSDVLYLIMPDRFANGNPSNDIIPGMLEGNVDRNEPFARHGGDLKGIENHLDYIADL
GVTSIWLNPIQENDMKEGSYHGYAITDYYQVDRRFGSNEEFRKLTQEANAKGLKVVMDMI
FNHCGSDNYLFKDMPSKDWFNFEGNYVQTSFKTATQMDPYASDYEKKIAIDGWFTLTMPD
FNQRNRHVATYLIQSSIWWIEYAGINGIRQDTHPYADFDMMARWCKAVNEEYPKFNIVGE
TWLGNNVLISYWQKDSRLAYPKNSNLPTVMDFPLMEEMNKAFDEETTEWNGGLFRLYEYL
SQDIVYSHPMSLLTFLDNHDTSRFYRSEADTKNLDRYKQALTFLLTTRGIPQIYYGTEIL
MAADKANGDGLLRCDFPGGWPNDTKNCFDAANRTPQQNEAFSFMQKLLQWRKGNEVIAKG
QLKHFAPNKGVYVYERKYGDKSVVVFLNGNDREQTIDLVPYQEILPASSAFDLLTEKKVE
LRNELTLPSREIYLLSF