Protein Info for BT3567 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): periplasmic beta-1,2-glucosidase / beta-1,3-glucosidase
Rationale: Specifically important in carbon source Laminaribiose (beta-D-glucose-(1->3)-D-glucose). Cleaves beta,1-2 and beta,1-3 linkages and has little activity on cellobiose (PMID:28343388).
Original annotation: periplasmic beta-glucosidase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 771 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 41 to 349 (309 residues), 267.5 bits, see alignment E=2.8e-83 PF01915: Glyco_hydro_3_C" amino acids 391 to 654 (264 residues), 169.8 bits, see alignment E=1.1e-53 PF14310: Fn3-like" amino acids 691 to 760 (70 residues), 85.5 bits, see alignment E=3.4e-28

Best Hits

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to bth:BT_3567)

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1U1 at UniProt or InterPro

Protein Sequence (771 amino acids)

>BT3567 periplasmic beta-1,2-glucosidase / beta-1,3-glucosidase (Bacteroides thetaiotaomicron VPI-5482)
MINKKIFFSLLLLAAGFLSAAAQKSPQDMDRFIDALMKKMTVEEKIGQLNLPVTGEITTG
QAKSSDIAAKIKRGEVGGLFNLKGVEKIRDVQKQAVEQSRLGIPLLFGMDVIHGYETMFP
IPLGLSCTWDMTAIEESARIAAIEASADGISWTFSPMVDISRDPRWGRVSEGSGEDPFLG
AMIAEAMVLGYQGKDMQRNDEIMACVKHFALYGAGEGGRDYNTVDMSRQRMFNEYMLPYE
AAVEAGVGSVMASFNEVDGVPATANKWLMTDVLRGQWGFNGFVVTDYTGISEMIDHGIGD
LQTVSARAINAGVDMDMVSEGFVSTLKKSIQEGKVSMETLNTACRRILEAKYKLGLFDNP
YKYCDLKRPARDIFTKAHRDAARRIAAESFVLLKNDNVTLRPGTPAEPLLPFNPKGNIAV
IGPLADSRTNMPGTWSVAAVLDRCPSLVEGLKEMTAGKANILYAKGSNLISDASYEERAT
MFGRSLNRDNRTDEQLLNEALTVANQSDIIIAALGESSEMSGESSSRTDLNIPDVQQNLL
KELLKTGKPVVLVLFTGRPLTLTWEQEHVPAILNVWFGGSEAAYAIGDALFGYVNPGGKL
TMSFPKNVGQIPLYYAHKNTGRPLAQGKWFEKFRSNYLDVDNEPLYPFGYGLSYTTFSYG
DIDLSRSTIDMTGELTAAVMVTNTGTWPGSEVVQLYIRDLVGSTTRPVKELKGFQKIFLE
PGQSEIVRFKIAPEMLRYYNYDLQLVAEPGEFEVMIGTNSRDVKSARFTLK