Protein Info for BT3558 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative endonuclease (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 379 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF19190: BACON_2" amino acids 45 to 118 (74 residues), 32.8 bits, see alignment E=7.8e-12 PF13004: BACON" amino acids 62 to 117 (56 residues), 40.9 bits, see alignment 2.4e-14 PF01223: Endonuclease_NS" amino acids 147 to 362 (216 residues), 186.7 bits, see alignment E=7.4e-59

Best Hits

KEGG orthology group: K01173, endonuclease G, mitochondrial (inferred from 100% identity to bth:BT_3558)

Predicted SEED Role

"putative endonuclease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1V0 at UniProt or InterPro

Protein Sequence (379 amino acids)

>BT3558 putative endonuclease (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKYKFIQTLLLVLLPTLFAACGSDNNDPTDNIGGLSVSTDLKNNEVSAKGGSFFLQIKTD
GKWTASSQDSWCTINNKEGNGNASTICSVSANDDDERYTVITVTSNGKSENITITQKGGN
GEEPDPDPNPSGYAGRIEIPKLKGGNSNLFITHTTQYNGKEVITYSFEYDCTQKSSRWVA
FTFNTSTPDNNVGRAGDFSDDPSIPSQYRTHDGDYTGSGYSRGHLAASSDRQYSVAANKQ
TFYMSNMNPQIQNGFNGGIWASLEGKVQSWGKITNDQDTLYVAKGGTIDNNNIIKYLKAN
NTIPVPKYFYMAILSLKNGQYKAIGFWFEHKSYSNSSYAAYALSIDELEEKTGIDFFHNL
PDKIENEVERSYNKSDWGL