Protein Info for BT3516 in Bacteroides thetaiotaomicron VPI-5482

Annotation: arabinan endo-1,5-alpha-L-arabinosidase A precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF04616: Glyco_hydro_43" amino acids 31 to 312 (282 residues), 192.1 bits, see alignment E=1.5e-60 amino acids 337 to 620 (284 residues), 276.3 bits, see alignment E=3.1e-86 PF00251: Glyco_hydro_32N" amino acids 37 to 175 (139 residues), 23 bits, see alignment E=5.6e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_3516)

Predicted SEED Role

"Arabinan endo-1,5-alpha-L-arabinosidase (EC 3.2.1.99)" in subsystem L-Arabinose utilization (EC 3.2.1.99)

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.99

Use Curated BLAST to search for 3.2.1.99

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A1Z2 at UniProt or InterPro

Protein Sequence (637 amino acids)

>BT3516 arabinan endo-1,5-alpha-L-arabinosidase A precursor (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKLRHLFLFCVICCSVSISAQNKSKLPKTSGNPIFPGWYADPEGIVFGDEYWIYPTYSAA
YDDQIFMDAFSSKDLVNWTKHPKVLSKENISWLRRALWAPAVIHANDKYYFFFGANDIQN
NNELGGIGVAVADNPAGPFKDALGKPLIDKIVNGAQPIDQFVFKDDDGQYYMYYGGWGHC
NMVKMAPDLLSIVPFEDGTIYKEVTPQNYVEGPFMLKHNGKYYFMWSEGGWGGPDYSVAY
AIADSPFGPFERVGKILQQDTNIATGAGHHSVVKGPGSDEWYIIYHRRPLGETAANSRAT
CIDRMYFNKDGKIEPVRMTFEGVGASPLATPVRAKTYANPVINFSLPDPTIIKGDDEYYY
LYATESIKNVPIHRSRDLVNWYYVGTAFTDATRPNFEPKGRIWAPDINKIGDKYVMYYSM
STWGGEWTCGIGIATADKPEGPFTDLGKLFRSNEINIQNCIDPFYIEDNGKKYLFWGSFH
GIYGTELTDDGLSLKKGAPIEQIAGTAYEGTYIHKRDGYYYLFASIGRCCEGLKSTYTTV
VGRSKNLFGPYVDKKGRSMSDNHHEILIQKNKAFVGPGHNSELVTDKKGNDWMFYHAVSV
ANPEGRVLMMDRVQWKGGWPFVKGAVPSLETAAPDFR