Protein Info for BT3331 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): Outer membrane binding protein for chondroitin sulfate and hyaluronic acid (susD-like)
Rationale: Specifically important in carbon source Hyaluronic acid sodium salt from Streptococcus equi; carbon source Chondroitin sulfate A sodium salt from bovine trachea. The adjacent susC-like protein BT3332 has similar phenotypes.
Original annotation: putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 572 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF14322: SusD-like_3" amino acids 21 to 222 (202 residues), 78 bits, see alignment E=1.8e-25 PF12771: SusD-like_2" amino acids 95 to 192 (98 residues), 32.9 bits, see alignment E=5.5e-12 PF07980: SusD_RagB" amino acids 282 to 572 (291 residues), 209.1 bits, see alignment E=1.7e-65

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_3331)

Predicted SEED Role

"Putative outer membrane protein, probably involved in nutrient binding"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A2H4 at UniProt or InterPro

Protein Sequence (572 amino acids)

>BT3331 Outer membrane binding protein for chondroitin sulfate and hyaluronic acid (susD-like) (Bacteroides thetaiotaomicron VPI-5482)
MKALKITIIALLAGFSMVSCDFLDKEPTKLTPENYFNTPAEANSFLTGIYAILSQPTFYG
GDYMYLVAGDDLSHYGGSGRGPASTGLICNNATTSDNAVTAFWYALYSGINRANMFLENI
DKVNGFDAGVKEQYIAEARFLRAFYYFNLVECWGDVPFKTVSTQSVTNLNIPRTDKQEIY
DFIISEMADAAETGLKSASDLAYKPGRISQSTAWGILARVYLFRAGEHYREGRNATQAEK
KDYFERASFYAQKVMTAGHKLAANYWDPFIDMCSDKYNTTANESIWEAEFAGNNTSDTQA
EGRIGNIIGLAGPDLSSKSDVTGAKDPGYGYAFIYSTPKLYNLYVNNGDTKRFNWSIAPF
EYKEAGGKNTGVTHREFEQGKLAEVMSQYGQQRGTYQYADDTEKTTATKNFSRMCGKYRR
EYEADKKDKNYTSINFPILRYADVLLMIAEAENEANNGPTTLAYQCMKEVRERAGLNELP
DMTQEEFRQTVKDERAMELCFEYTRRFDLIRWGEYVKNMRALVTEAQSGNNWTQGPTNVY
TYFNISSTYNYFPIPDAEMSVNKDITQNNPGW