Protein Info for BT3082 in Bacteroides thetaiotaomicron VPI-5482

Annotation: 2,6-beta-D-fructofuranosidase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00251: Glyco_hydro_32N" amino acids 121 to 401 (281 residues), 246 bits, see alignment E=7.1e-77 PF08244: Glyco_hydro_32C" amino acids 412 to 541 (130 residues), 28.1 bits, see alignment E=1.9e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_3082)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.B3

Use Curated BLAST to search for 3.2.1.B3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A373 at UniProt or InterPro

Protein Sequence (548 amino acids)

>BT3082 2,6-beta-D-fructofuranosidase precursor (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKTLSRTVLTLFVLSFSCLAAIAGEVSFKITKQYLNFPISHKENRGRMTFEVNGKPDLSV
VIRLAPDEAEYWVFKDVSAFKGKTIKITYDGNEKGLSKIYQSDEIEGQAELYKEKNRPQF
HFTTRRGWINDPNGLIYYEGEYHLFYQHNPFERDWENMHWGHAVSKDLIHWTELPDALYP
DHLGTMFSGSAVIDYDNTAGFNKGKTPAMVAAFTAASSDRQVQGIAYSLDKGRTFTKYDK
NPVINSKEKWNSQDTRDPKVFWYAPSKHWVLVLNERDGHSIYTSSNLKDWKYESHVTGFW
ECPELFELPVDGDKNHTKWVMYGATGTYMLGSFDGKVFTPEAGKYCYTTGSIYAAQTFTN
IPASDGRRIQIGWGRISHPGMPFNGMMMLPTELTLRTTKDGIRLVNVPVKEVESLCKPLR
SWKSLSSDEANRHLKEFYDADCLRIKTTIKLSHATDAGFNLFGQRMIGYDMNSNTLNGRF
YSPQDPTSMELSADIYIDRTSIEVFIDGGLYSYSMERRPDTNNREGLHFWGNRIEVKDLQ
VFSVESIW