Protein Info for BT2855 in Bacteroides thetaiotaomicron VPI-5482

Annotation: beta-mannosidase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 989 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF13364: BetaGal_ABD2" amino acids 98 to 177 (80 residues), 22.8 bits, see alignment E=4.6e-08 PF00703: Glyco_hydro_2" amino acids 224 to 338 (115 residues), 62.8 bits, see alignment E=1.7e-20 PF00754: F5_F8_type_C" amino acids 737 to 844 (108 residues), 80.4 bits, see alignment E=4.6e-26 PF18368: Ig_GlcNase" amino acids 879 to 988 (110 residues), 85.4 bits, see alignment E=1e-27 PF17753: Ig_mannosidase" amino acids 922 to 970 (49 residues), 22.9 bits, see alignment 2e-08

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_2855)

Predicted SEED Role

"Beta-mannosidase (EC 3.2.1.25)" in subsystem Mannose Metabolism (EC 3.2.1.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.25

Use Curated BLAST to search for 3.2.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A3U9 at UniProt or InterPro

Protein Sequence (989 amino acids)

>BT2855 beta-mannosidase precursor (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MCMKFIKKKGKSLGIGLAMVGSLLVSSLQAGPVYLHSGESIVSWKLKPEAEVGTNVPALL
NAGYDTSSWVDAVVPGTAFTSYVTAGLEKDPNFGDNIHNVDRAKYDRSFWYRTEFKVPAD
FDKALTWLNFNGVNRKAEIYLNGHLLGILDGFMHRGRFNITEIVKKDQPNVLAVLVHMPQ
TPLANYGSPTYLSSGGWDWMPYVPGLNMGITDKVYLSNTGTATIIDPWIRTDLPLPSRTR
ADLSVALEVKNSSDKPSKVVVNGTITPGDVKFTKEVDINPGATSEVKFDKRYYPQLVINS
PKLWWPNGYGEPNLYTCKLEVSVDGKVSETKDVTFGIKEYSYDTNNNTLHLHINGVPVFV
KGANWGMSEYMLRCRGEEYDTKLRFHHEMNFNMIRNWLGSTTDDEFYEMCDKYGLMVWDD
FWINSNPNLPYDLNAFNNNMIEKIKRVRNHPSLAVWCGDNESNPQPPLEGWMAENIKTFD
GGDRYFQPNSHAGNLTGSGPWGAFDPRFYFTEYPDGLEGDPERGWGFRTEIGTAVVPTFE
SFKKFMPEKDWWPRNKMWDLHYFGQSAFNAAPDRYDASLAKGFGAPSGIEDYCRKAQLIN
IESNKAMYEGWLDRMWDDASGIMTWMGQSAYPSMVWQTYDYYYDLTGAYWGTKSACEPLH
ILWNPVTDAVKVANTTAENYQDLKAEVTVYNMDGKAVPAYSKSSVVHSASNSTLECFTID
FNKERPNLGLNQKVVVSSTSEGDPSMAVDGKKDTRWSSAYRDNEWIYVDLGKVQPVGGVR
LDWEASYGKEYKIQVSNDAQQWEEAYSTKNGIGGVELITFPEKDARYVRMFGFKRGWWYG
YSLWSFDVLGGTGKSEGLSDVHFIRLKLTDKNGKLISENNYWRGNDRRDFTALNQLPKAE
LKVSSKMEQKGEKAEIRATIGLPKSAKSVAFAVHVQAVRTVDGERILPAIMNDNYFTLMP
GENKEITINFDKSLLKGGSYKLLVTPYNN