Protein Info for BT2827 in Bacteroides thetaiotaomicron VPI-5482

Annotation: DNA topoisomerase I (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 782 TIGR01051: DNA topoisomerase I" amino acids 5 to 487 (483 residues), 628.4 bits, see alignment E=1e-192 PF01751: Toprim" amino acids 5 to 113 (109 residues), 78.3 bits, see alignment E=6e-26 PF01131: Topoisom_bac" amino acids 128 to 487 (360 residues), 369.5 bits, see alignment E=3.7e-114 amino acids 517 to 568 (52 residues), 38.9 bits, see alignment 8.9e-14 PF13368: Toprim_C_rpt" amino acids 599 to 656 (58 residues), 54.6 bits, see alignment 1.3e-18 amino acids 660 to 709 (50 residues), 62.8 bits, see alignment 3.7e-21 amino acids 719 to 767 (49 residues), 44 bits, see alignment 2.7e-15

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 100% identity to bth:BT_2827)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A3X7 at UniProt or InterPro

Protein Sequence (782 amino acids)

>BT2827 DNA topoisomerase I (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MQKNLVIVESPAKAKTIEKFLGKDFKVLSSYGHIRDLKKKEFSIDVDKNFKPDYEIPADK
KALVSTLKAEAKEAETVWLASDEDREGEAIAWHLYEVLKLKPENTKRIVFHEITKGAILK
AIEQPRNIDLNLVNAQQARRILDRIVGFELSPVLWRKVKPSLSAGRVQSVAVRLIVERER
EIHAFKSEAAYRVIAIFLVPDTDGKLVEMKAELSRRIKTKEEAKAFLDACKGATFTIEDI
TTRPVKKTPPAPFTTSTLQQEAARKLGYTVAQTMMIAQRLYESGFITYMRTDSVNLSEYA
TASSKDAIIQMMGERYVHPRHFETKTKGAQEAHEAIRPTYMENQSIEGTAQEKKLYDLIW
KRTIASQMADAELEKTTATISISKSGDAFTAIGEVIKFDGFLRVYRESYDDENEQEDESR
LLPPLKKGQKLEYGPIVATERFTQRPPRYTEASLVRKLEELGIGRPSTYAPTISTIQQRE
YVEKGNKDGEERTFNVLTLKDNQIKDESHNEVTGAEKSKLFPTDTGTVVNDFLTEYFPDI
LDYNFTASVEKEFDEIAEGEVKWTSIMKTFYDQFHPAVEKTLSIKTEHKVGERMLGEEPE
TGKPVSVKIGRFGPVVQIGAADDEEKPRFAQMKKGQSMETITLEEALELFKLPRTLGEYE
GKTVTVGIGRFGPYIQHNKVYVSLPKTLDPMKVTLEEAEQLILEKRTKEAERHIKKFDEE
PELEILNGRYGPYIAYKGNNYKIPKDIVPQDLSLKSCFDLIKIQDEKGPGTSAKGKRTAK
KK