Protein Info for BT2803 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): ribokinase (EC 2.7.1.15)
Rationale: Specifically important in carbon source D-Ribose. An adjacent protein (BT2804) is also expected to be a ribokinase and is also required. It is not clear why both proteins are required.
Original annotation: ribokinase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF00294: PfkB" amino acids 24 to 314 (291 residues), 238.6 bits, see alignment E=1.1e-74 TIGR02152: ribokinase" amino acids 25 to 319 (295 residues), 396.7 bits, see alignment E=3.5e-123 PF08543: Phos_pyr_kin" amino acids 174 to 302 (129 residues), 50.3 bits, see alignment E=2.3e-17

Best Hits

Swiss-Prot: 46% identical to RBSK_HAEIN: Ribokinase (rbsK) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K00852, ribokinase [EC: 2.7.1.15] (inferred from 100% identity to bth:BT_2803)

Predicted SEED Role

"Ribokinase (EC 2.7.1.15)" in subsystem D-ribose utilization or Deoxyribose and Deoxynucleoside Catabolism (EC 2.7.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.15

Use Curated BLAST to search for 2.7.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A401 at UniProt or InterPro

Protein Sequence (321 amino acids)

>BT2803 ribokinase (EC 2.7.1.15) (Bacteroides thetaiotaomicron VPI-5482)
MIIAFICIVLIFNTMETISIHRPKIVVIGSCNTDMVVKANRLPVPGETILGGTFYMNPGG
KGANQAIAAARLGAEVTFISKIGYDLFGLQALEIYRSEKINTEYIFTDQKSPSGVALISV
DSFGENSIIVAPGASRSLSIEDINKAEEKIKEADIILMQLEIPIDTVEYAATIACKYGKK
VILNPAPASSLSNSFLMNVHTILPNRIEAEMLSGIKVMNIESAHRAAQAIGEKGIENVVI
TLGKDGAYVKEKDEYTMIPAKEVETIDTTGAGDVFCGAFSVCLSEGHSLAKSVKFANAAA
AIAVTRIGAQSAIPYKREVVL