Protein Info for BT2734 in Bacteroides thetaiotaomicron VPI-5482

Annotation: DNA-directed RNA polymerase beta chain (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1270 TIGR02013: DNA-directed RNA polymerase, beta subunit" amino acids 7 to 1269 (1263 residues), 1861.1 bits, see alignment E=0 PF04563: RNA_pol_Rpb2_1" amino acids 25 to 131 (107 residues), 34.9 bits, see alignment E=3.7e-12 amino acids 133 to 461 (329 residues), 33.4 bits, see alignment E=1.1e-11 PF04561: RNA_pol_Rpb2_2" amino acids 149 to 219 (71 residues), 32.7 bits, see alignment 1.8e-11 amino acids 294 to 415 (122 residues), 75.9 bits, see alignment E=1e-24 PF04565: RNA_pol_Rpb2_3" amino acids 474 to 542 (69 residues), 98.9 bits, see alignment 4.3e-32 PF10385: RNA_pol_Rpb2_45" amino acids 552 to 620 (69 residues), 87.5 bits, see alignment 1.5e-28 PF00562: RNA_pol_Rpb2_6" amino acids 683 to 1194 (512 residues), 407.7 bits, see alignment E=1.5e-125 PF04560: RNA_pol_Rpb2_7" amino acids 1196 to 1269 (74 residues), 88 bits, see alignment 1.4e-28

Best Hits

Predicted SEED Role

"DNA-directed RNA polymerase beta subunit (EC 2.7.7.6)" in subsystem RNA polymerase bacterial (EC 2.7.7.6)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.6

Use Curated BLAST to search for 2.7.7.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A469 at UniProt or InterPro

Protein Sequence (1270 amino acids)

>BT2734 DNA-directed RNA polymerase beta chain (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MSSNTVNQRVNFASTKNPLEYPDFLEVQLKSFQDFLQLDTPPEKRKNEGLYKVFAENFPI
ADTRNNFVLEFLDYYIDPPRYTIDDCIERGLTYSVPLKAKLKLYCTDPDHEDFDTVIQDV
FLGPIPYMTDKATFVINGAERVVVSQLHRSPGVFFGQSVHANGTKLYSARIIPFKGSWIE
FATDINNVMYAYIDRKKKLPVTTLLRAIGFENDKDILEIFNLAEDVKVNKTNLKRVLGRK
LAARVLKTWIEDFVDEDTGEVVSIERNEVIIDRETVLEEVHIDEILESGVQNILLHKDEP
NQSDFSIIYNTLQKDPSNSEKEAVLYIYRQLRNADPADDASAREVINNLFFSEKRYDLGD
VGRYRINKKLNLTTDMDVRVLTKEDIIEIIKYLIELINSKADVDDIDHLSNRRVRTVGEQ
LSNQFAVGLARMSRTIRERMNVRDNEVFTPIDLINAKTISSVINSFFGTNALSQFMDQTN
PLAEITHKRRMSALGPGGLSRERAGFEVRDVHYTHYGRLCPIETPEGPNIGLISSLCVFA
KINELGFIETPYRKVENGKVDLSDNGLVYLTAEEEEEKIIAQGNAPLNDDGTFVRNKVKS
RQDADFPVVEPSEVDLMDVSPQQIASIAASLIPFLEHDDANRALMGSNMMRQAVPLLRSE
APIVGTGIERQLVRDSRTQITAEGDGVVDFVDATTIRILYDRTEDEEFVSFEPALKEYRI
PKFRKTNQNMTIDLRPICDKGQRVKKGDILTEGYSTEKGELALGKNLLVAYMPWKGYNYE
DAIVLNERVVREDLLTSVHVEEYSLEVRETKRGMEELTSDIPNVSEEATKDLDENGIVRI
GARIEPGDIMIGKITPKGESDPSPEEKLLRAIFGDKAGDVKDASLKASPSLKGVVIDKKL
FSRVIKNRSSKLADKALLPKIDDEFESKVADLKRILVKKLMTLTEGKVSQGVKDYLGAEV
IAKGSKFSASDFDSLDFTSIQLSNWTSDDHTNGMVRDLVMNFIKKYKELDAELKRKKFAI
TIGDELPAGIIQMAKVYIAKKRKIGVGDKMAGRHGNKGIVSRVVRQEDMPFLADGTPVDI
VLNPLGVPSRMNIGQIFEAVLGRAGKTLGVKFATPIFDGATMDDLDQWTDKAGLPRYCKT
YLCDGGTGEQFDQAATVGVTYMLKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRF
GEMEVWALEGFGAAHILQEILTIKSDDVVGRSKAYEAIVKGEPMPQPGIPESLNVLLHEL
RGLGLSINLE