Protein Info for BT2623 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative alpha-1,6-mannanase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF03663: Glyco_hydro_76" amino acids 156 to 503 (348 residues), 156.3 bits, see alignment E=2.5e-49 PF07470: Glyco_hydro_88" amino acids 175 to 360 (186 residues), 37.5 bits, see alignment E=2.2e-13

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_2623)

MetaCyc: 100% identical to endo-1,6-alpha-mannanase (Bacteroides thetaiotaomicron)
Mannan endo-1,6-alpha-mannosidase. [EC: 3.2.1.101]

Predicted SEED Role

"putative alpha-1,6-mannanase"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.101

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A4H6 at UniProt or InterPro

Protein Sequence (517 amino acids)

>BT2623 putative alpha-1,6-mannanase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKKVIKKYFFLALAIIMYSCNEDEKYDILERYTPETITSDEIAPVLNLQAQYMDSNSEIV
LVTWMNPEDDFLSKVEISCCSANDNLLGEPVLLDAVSTKVGSYQTSLSVEERGYVKIVAI
NEKGVRSEARTAEILSSQQDFVYRADCLMSSVIELFFGGRYNAWNENYPNATGPYWDGIA
AVWGQGAAYSGFVTMYKVTKETNNEKLRAKYAEKEETFLNSIDIFLNNGSGRKSFAYGTY
IGPNDERYYDDNVWIGIEMANLYELTGNEVYLQHANTVWNFILEGIDDVTGGGVYWKEGA
VSKHTCSTAPAAVMALKLYQLSKNESYLEIAKSLYSYCKDVLQDPNDYLFYDNVRLSDPS
DKNSELKVSKDKFTYNSGQPMLAAAMLYRITKEEQFLKDAQNIAQSIYKKWFKNYHSSIL
DRDIMILSDPNTWFNAVMFRGFVELYKIDKNDVYVKAVKNTMEHAWQSNCRNRLTNLMSD
DYAGDKKEGKWNIKTQGAFVEIFSLIGELEQLGCFQE