Protein Info for BT2493 in Bacteroides thetaiotaomicron VPI-5482

Annotation: ROK family transcriptional repressor, with glucokinase domain (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00480: ROK
  • amino acids 11 to 318 (308 residues), 208.9 bits, E=1.2e-65

Best Hits

KEGG orthology group: K00845, glucokinase [EC: 2.7.1.2] (inferred from 100% identity to bth:BT_2493)

MetaCyc: 92% identical to glucokinase / N-acetylglucosamine kinase / mannokinase (Bacteroides fragilis)
Mannokinase. [EC: 2.7.1.7]; N-acetylglucosamine kinase. [EC: 2.7.1.7, 2.7.1.59]; Hexokinase. [EC: 2.7.1.7, 2.7.1.59, 2.7.1.1, 2.7.1.2]

Predicted SEED Role

"Glucokinase (EC 2.7.1.2)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis (EC 2.7.1.2)

MetaCyc Pathways

• glycolysis III (from glucose) (11/11 steps found)
• homolactic fermentation (11/12 steps found)
• glycogen degradation I (7/8 steps found)
• Bifidobacterium shunt (12/15 steps found)
• CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis (4/4 steps found)
• heterolactic fermentation (14/18 steps found)
• N-acetylglucosamine degradation II (3/3 steps found)
• D-mannose degradation II (2/2 steps found)
• mannitol degradation II (3/4 steps found)
• sucrose degradation III (sucrose invertase) (3/4 steps found)
• GDP-α-D-glucose biosynthesis (2/3 steps found)
• trehalose degradation IV (2/3 steps found)
• trehalose degradation V (2/3 steps found)
• glycogen degradation II (4/6 steps found)
• trehalose degradation I (low osmolarity) (1/2 steps found)
• trehalose degradation II (cytosolic) (1/2 steps found)
• 1,3-propanediol biosynthesis (engineered) (6/9 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• glucose and glucose-1-phosphate degradation (3/5 steps found)
• sucrose biosynthesis II (5/8 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (3/6 steps found)
• chitin biosynthesis (4/9 steps found)
• chitin derivatives degradation (3/8 steps found)
• superpathway of CMP-sialic acids biosynthesis (6/15 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.1, 2.7.1.2

Use Curated BLAST to search for 2.7.1.1 or 2.7.1.2 or 2.7.1.59 or 2.7.1.7

Sequence Analysis Tools

See Q8A4V4 at UniProt or InterPro

Protein Sequence (326 amino acids)

>BT2493 ROK family transcriptional repressor, with glucokinase domain (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNSNMEKPYVVGIDIGGTNTVFGIVDARGTIIASSSIKTAGYPTAAEYADEVCKNLLPLI
IANGGVDKIRGIGVGAPNGNYYTGTIEFAPNLPWKGILPLAAMFEERLGIPTALTNDANA
AAIGEMTYGAARGMKDFIMITLGTGVGSGIVINGQMVYGHDGFAGELGHVIARRDGRVCG
CGRKGCLETYCSATGVARTAREFLAARTDASLLRNIPAENITSKDVYDAAVQGDKLAQEI
FEFTGNILGEALADAIAFSSPEAIVLFGGLAKSGDYIMKPIQKAIDDNILNIYKGKTKLL
VSELKDSDAAVLGASALAWELKDLKE