Protein Info for BT2416 in Bacteroides thetaiotaomicron VPI-5482

Annotation: GTP cyclohydrolase II (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase" amino acids 6 to 202 (197 residues), 241.2 bits, see alignment E=7.4e-76 PF00926: DHBP_synthase" amino acids 10 to 201 (192 residues), 278.5 bits, see alignment E=2.4e-87 PF00925: GTP_cyclohydro2" amino acids 212 to 376 (165 residues), 242.7 bits, see alignment E=1.5e-76 TIGR00505: GTP cyclohydrolase II" amino acids 213 to 400 (188 residues), 245.9 bits, see alignment E=2.3e-77

Best Hits

Swiss-Prot: 100% identical to RIBBA_BACTN: Riboflavin biosynthesis protein RibBA (ribBA) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 100% identity to bth:BT_2416)

MetaCyc: 49% identical to GTP cyclohydrolase II (Chlamydia trachomatis)
GTP cyclohydrolase II. [EC: 3.5.4.25]

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A528 at UniProt or InterPro

Protein Sequence (404 amino acids)

>BT2416 GTP cyclohydrolase II (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MEEIKMDRIEDAIADFKEGKFVIVVDDEDRENEGDLIIAAEKITPEKVNFMLKHARGVLC
APITVSRCKELDLPHQVSDNTSVLGTPFTVTIDKLEGCSTGVSASDRAATIQALADPAST
PATFGRPGHINPLYAQEKGVLRRAGHTEATIDMARLAGLYPAGALMEIMSDDGTMARLPE
LRAMADEHGLKLISIHDLIAYRLKQESIVEKGVEVNMPTEHGMFRLIPFRQKSNGLEHMA
IFKGTWNEDEPILVRVHSSCATGDILGSHRCDCGEQLHKAMEMIEKEGKGVVVYLNQEGR
GIGLMEKMKAYKLQEDGMDTVDANLCLGHLADERDYGVGAQILRELGVHKMKLLTNNPVK
RVGLEAYGLEIVENVPVETVPNPYNERYLRTKKERMGHTLHFNK