Protein Info for BT2324 in Bacteroides thetaiotaomicron VPI-5482

Annotation: DNA topoisomerase III (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 transmembrane" amino acids 32 to 48 (17 residues), see Phobius details PF01751: Toprim" amino acids 3 to 139 (137 residues), 40.5 bits, see alignment E=3.9e-14 PF01131: Topoisom_bac" amino acids 157 to 575 (419 residues), 412 bits, see alignment E=4.7e-127 PF13342: Toprim_Crpt" amino acids 627 to 686 (60 residues), 67.5 bits, see alignment 1.2e-22

Best Hits

KEGG orthology group: K03169, DNA topoisomerase III [EC: 5.99.1.2] (inferred from 100% identity to bth:BT_2324)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A5B5 at UniProt or InterPro

Protein Sequence (699 amino acids)

>BT2324 DNA topoisomerase III (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKVIIAEKPSVAQAIASVVGAHQRKEGYLMGGGYAVAWAFGHLVGLAMPESYGLSGFRRE
HLPILPQEFRLVPRQVREGKAYKDDPGVVRQLGVIRDLFDRCESIIVATDAGREGELIFR
YVYNYLGCTKPFARLWISSLTDKAIREGLQNLREGSLYDNLYLSAKARSEADWLVGINSS
QALSLAAGQGVFSLGRVQTPTLAMICSRYRENRQFEPRTYFRIKVSAAKDGIDFSALSQN
RFDDLDSATARLREIKDGCMLAVTSVERREAVQEPPLLYDLTALQKEANSKLDFSADKTL
TLAQSLYEKKVLSYPRTGSRYISEDVFDEIPSRIMLLRQYPRFTATASVLPDAPLNRRPV
DDAKVTDHHALLITENLPEGLSQDEQAIYEMVAARLLEAFLPCCVKEMTEVALSAGGEIF
TLKGAVIKSAGWRAVRGEPEEEAEETTLPGLQTGETLPLLASETVEKQTKPQPLHTESSL
LSAMEHCGKEISDEQLRAGIREIGIGTPATRAAVIETLFARDYIRRDKKNLVPTEKGLAV
YDTVKDKKIADVEMTAAWENTLAKIETGEADALSFRRNIEVYTAQIVTELLAATLNVAPS
GEQCTCPKCRKSRILFFDKVAKCADVDCSFTLFRNKGGKILTDGQIIELATKGRTPLVKG
FKSREGKSFDAALVLNPDFTIGYSFPDRKPQEKKSGRKK