Protein Info for BT2252 in Bacteroides thetaiotaomicron VPI-5482

Annotation: multidrug efflux membrane fusion protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1010 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 336 to 352 (17 residues), see Phobius details amino acids 359 to 379 (21 residues), see Phobius details amino acids 385 to 410 (26 residues), see Phobius details amino acids 430 to 450 (21 residues), see Phobius details amino acids 462 to 485 (24 residues), see Phobius details amino acids 525 to 545 (21 residues), see Phobius details amino acids 844 to 864 (21 residues), see Phobius details amino acids 871 to 891 (21 residues), see Phobius details amino acids 897 to 921 (25 residues), see Phobius details amino acids 943 to 963 (21 residues), see Phobius details amino acids 975 to 1000 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1000 (998 residues), 970 bits, see alignment E=1.4e-295 PF03176: MMPL" amino acids 332 to 487 (156 residues), 29.6 bits, see alignment E=3.6e-11 amino acids 809 to 1003 (195 residues), 41 bits, see alignment E=1.2e-14

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 100% identity to bth:BT_2252)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A5I7 at UniProt or InterPro

Protein Sequence (1010 amino acids)

>BT2252 multidrug efflux membrane fusion protein (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNISELSIRRPVLATVLTIIILLFGFIGYSYLGVREYPSVDNPIISVSCSYPGANADVIE
NQITEPLEQNINGIPGIRSLSSVSQQGQSRITVEFELSVDLETAANDVRDKVSRAQRYLP
RDCDPPTVSKADADAMPILMVALQSDKRSLLELSEIADLTVKEQLQTISDVSSVSIWGEK
RYSMRLWLDPVKMAGYGITPIDVKNAVDNENVELPSGSIEGNTTELTIRTLGLMHTADEF
NNLIVKEDNNRIVRFSDIGRAELGPADIKSYMKMNGVPMVGVVVIPQPGANHIEIADAVY
KRMEQMKKDLPEDVTYSYGFDNTKFIRASINEVKETVYVAFILVIIIIFLFLRDWRVTLV
PCIVIPVSLIGAFFVMYLAGFSINVLSMLAIVLAVGLVVDDAIVMTENIYIRIEKGMSPK
EAGIEGAKEIFFAVISTTITLVAVFFPIVFMQGMTGRLFREFSIVISGSVIISSFAALTF
TPMLATKLLIKREKQNWFYTKTEPFFEGMNRWYSRSLAAFLRKRWLALPFTFITICLIGF
LWNAIPAEMAPLEDRSQISINTRGAEGVTYEYIRDYTEDINQLVDSILPDAEAVTARVSS
GSGNVRITLKDMKDRDYTQMEVAEKISKAVQKKTMARSFVQQQSSFGGRRGSMPVQYVLQ
ATNLEKLQEVLPQFMAKVYENPVFQMADVDLKFSKPEARIRINRDKASVMGVSTKNIAQT
LQYGLSGQRMGYFYMNGKQYEILGEINRQQRNKPADLKAIYIRSSNGDMIQLDNLIELEN
GIAPPKLYRYNRFVSATISAGLADGKTIGQGLDEMDKIAKETLDDTFRTALSGDSKEYRE
SSSSLMFAFILAILLIYLILAAQFESFKDPLIIMLTVPLAIAGALVFMYFGDITMNIFSQ
IGIIMLIGLVAKNGILIVEFANQKQEAGEDKMSAIKDAALQRLRPILMTSASTVLGLIPL
AFASGEGANQRIAMGTAVVGGMVVSTLLTMYIVPAIYSYISTNRIKITKE