Protein Info for BT2158 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): glycoside 3-dehydrogenase (EC 1.1.99.13)
Rationale: This is a glycoside 3-dehydrogenase (PMID:38898276). It was difficult to map insertions in this gene because of a paralog, but reexamination of the data suggests that this gene has a similar fitness pattern as the surrounding genes and is important for growth on trehalose, leucrose, palatinose, and raffinose. And a clean deletion mutant failed to grow on trehalose. It has a TAT (twin arginine transport) signal and is probably exported together with its NAD(P) cofactor, as with the glucose:fructose oxidoreductase Gfo. It's unclear what the electron acceptor could be, given that this enzyme is in the periplasm and B.theta has a very limited electron transport chain. After cleavage by BT2157 (or BT2156, see PMID:38898276) and hydration by BT2157, the resulting 3-ketoglucose is reduced by BT2159, which appears to be cytoplasmic.
Original annotation: putative dehydrogenases and related proteins (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01408: GFO_IDH_MocA
  • amino acids 42 to 159 (118 residues), 54.9 bits, E=1.5e-18
• PF19051: GFO_IDH_MocA_C2
  • amino acids 205 to 255 (51 residues), 38.5 bits, 9.6e-14

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_4448)

MetaCyc: 100% identical to disaccharide 3-dehydrogenase BT2158 (Bacteroides thetaiotaomicron)
Glucoside 3-dehydrogenase. [EC: 1.1.99.13]; 1.1.99.13 [EC: 1.1.99.13]; 1.1.99.13 [EC: 1.1.99.13]; 1.1.99.13 [EC: 1.1.99.13]

Predicted SEED Role

"Myo-inositol 2-dehydrogenase (EC 1.1.1.18)" in subsystem Inositol catabolism (EC 1.1.1.18)

MetaCyc Pathways

• myo-inositol degradation II (1/5 steps found)
• myo-inositol degradation I (1/7 steps found)
• myo-, chiro- and scyllo-inositol degradation (1/10 steps found)
• streptomycin biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.18

Use Curated BLAST to search for 1.1.1.18 or 1.1.99.13

Sequence Analysis Tools

See Q8A5T0 at UniProt or InterPro

Protein Sequence (491 amino acids)

>BT2158 glycoside 3-dehydrogenase (EC 1.1.99.13) (Bacteroides thetaiotaomicron VPI-5482)
MSDFSRRKFLKTGAAALAGITIAPSSILGMSHGHVSPTDKLNLAAVGIGGMGHANINNVK
GTENIVALCDVDWKYAKGVFDEFPNAKKYWDYRKMYDEMGKSIDGVIIATADHTHAIITA
DAMTMGKHVYCQKPLTHSVYESRLLTKLAASTGVVTQMGNQGASGEGTDLVCEWIWNGEI
GEVTKVECATDRPIWPQGLNAPEKADRIPNTLNWDLFTGPAKLNPYNNVYHPWNWRGWWD
YGTGALGDMACHILHQPFRALKLGYPTKVEGSSTLLLSACAPQAQHVKMIFPARDNMPKV
ALPEVEVHWYDGGMMPERPKGFPEGKQLMGPGGGLTIFHGTKDTLICGCYGEQPFLLSGR
VPNAPKVCRRVTCSHEMDWVRACKEDKSNRVMPKADFSESGPMNEMVVMGVLAIRLQGLN
KTLEWDGANMCFTNIGDNETLRTCIKDGFTIHDGHPSFNKTWTDPINAKQFAAELVKHNY
REGWKLPDMPR