Protein Info for BT1915 in Bacteroides thetaiotaomicron VPI-5482

Annotation: pyruvate carboxylase subunit A (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 166.2 bits, E=7.9e-53
• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 445 (445 residues), 660.8 bits, E=5.2e-203
• PF02786: CPSase_L_D2
  • amino acids 115 to 322 (208 residues), 265.3 bits, E=9.3e-83
• PF02222: ATP-grasp
  • amino acids 137 to 292 (156 residues), 38.7 bits, E=2e-13
• PF07478: Dala_Dala_lig_C
  • amino acids 140 to 291 (152 residues), 40.7 bits, E=4.8e-14
• PF02785: Biotin_carb_C
  • amino acids 336 to 442 (107 residues), 138.9 bits, E=1.7e-44

Best Hits

Swiss-Prot: 52% identical to PYCA_METJA: Pyruvate carboxylase subunit A (pycA) from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to bth:BT_1915)

MetaCyc: 55% identical to pyruvate carboxylase subunit A (Methanothermobacter thermautotrophicus Delta H)
Pyruvate carboxylase. [EC: 6.4.1.1]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• gluconeogenesis III (10/12 steps found)
• reductive TCA cycle I (9/11 steps found)
• incomplete reductive TCA cycle (6/7 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• biotin-carboxyl carrier protein assembly (3/4 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• jadomycin biosynthesis (3/9 steps found)
• 3-hydroxypropanoate cycle (5/13 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• glyoxylate assimilation (2/13 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (5/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (5/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)
• superpathway of mycolate biosynthesis (19/239 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Citrate cycle (TCA cycle)
• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.1, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.1 or 6.4.1.2

Sequence Analysis Tools

See Q8A6G9 at UniProt or InterPro

Protein Sequence (503 amino acids)

>BT1915 pyruvate carboxylase subunit A (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MIKKILVANRGEIAIRVMRSCREMEITSIAIFSEADRTAKHVLYADEAYCVGPAASKESY
LNIEKIIEVAKECHADAIHPGYGFLSENATFARRCQEENIIFIGPDPETMEAMGDKISAR
IKMIEAGVPVVPGTQENLKSVEEAVELCNKIGYPVMLKASMGGGGKGMRLIHSAEEVEEA
YTTAKSESLSSFGDDTVYLEKFVEEPHHIEFQILGDKHGNVIHLCERECSVQRRNQKIVE
ETPSVFVTPELRKDMGEKAVAAAKAVNYIGAGTIEFLVDKHRNYYFLEMNTRLQVEHPIT
EEVVGVDLVKEQIKVADGQVLQLKQKDIQQRGHAIECRICAEDTEMNFMPSPGIIKQITE
PNGIGVRIDSYVYEGYEIPIYYDPMIGKLIVWATNREYAIERMRRVLHEYKLTGVKNNIS
YLRAIMDTPDFVEGHYDTGFITKNGEHLQQCIMRTSERAENIAMIAAYMDYLMNLEENRG
DATDNRPISKWKEFGLHKGVLRI