Protein Info for BT1765 in Bacteroides thetaiotaomicron VPI-5482

Annotation: levanase precursor (2,6-beta-D-fructofuranosidase) (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 627 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF16352: DUF4980" amino acids 39 to 139 (101 residues), 128.4 bits, see alignment E=1.5e-41 PF00251: Glyco_hydro_32N" amino acids 140 to 441 (302 residues), 306.9 bits, see alignment E=3e-95 PF08244: Glyco_hydro_32C" amino acids 449 to 623 (175 residues), 112 bits, see alignment E=4.4e-36

Best Hits

KEGG orthology group: K01212, levanase [EC: 3.2.1.65] (inferred from 100% identity to bth:BT_1765)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.65, 3.2.1.B3

Use Curated BLAST to search for 3.2.1.65 or 3.2.1.B3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A6W1 at UniProt or InterPro

Protein Sequence (627 amino acids)

>BT1765 levanase precursor (2,6-beta-D-fructofuranosidase) (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKTTPWIKLCKGAVLALTVSFGLTYCQSTKSTFTLEQKGDSLTIVHITHPTNYILLPIEE
EEADESQVRLDTGNAADTDMDIRLAQTKVDYFVPFALPADTKVATLRIQKKSKDALCWEE
IKLSDTFDTTNTDKFRPVYHHTPLYGWMNDANGLVYKDGEYHLYYQYNPYGSKWGNMHWG
HSVSKDLMHWEHLAPAIARDTLGHIFSGSSIVDQENVAGYGAGSILAYYTSASDKNGQIQ
CLAYSKDNGRTFTKYEKNPVLRPSDGLKDFRDPKVFWYAPESKWVMIVSADKEMRFYDSH
NLKDWNYLSSFGEGYGVQPCQFECPDMVELPVDGDINYKKWALIVNVNPGCYFGGSATQY
FIGDFDGTKFICDNQPNVTKWLDWGKDHYATVCFSNTGDRVVAVPWMSNWQYCNIVPTRQ
FRSANALPRELGLYTQDNDIYLSAAPVAETKNLRKESKDVPSFTVDKDYHIESLLTDNEG
AYELSLNIEAGKAEIMGFSLFNDKGEKVDIYFNLPEKKLVMDRTKSGIVDFGKNSSPHEI
EAHDRRKTTSINYIDDFALATWAPIQKENEYKLDVFVDKCSVEIFLDGGKIAMTNLIFPT
EPYNRMCFYSKGGTFAVDSFSVYRLGL