Protein Info for BT1759 in Bacteroides thetaiotaomicron VPI-5482

Annotation: levanase precursor (2,6-beta-D-fructofuranosidase) (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 610 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF16352: DUF4980" amino acids 39 to 149 (111 residues), 127.7 bits, see alignment E=2.5e-41 PF00251: Glyco_hydro_32N" amino acids 150 to 440 (291 residues), 334.9 bits, see alignment E=9.3e-104 PF08244: Glyco_hydro_32C" amino acids 454 to 606 (153 residues), 127.1 bits, see alignment E=9.8e-41

Best Hits

KEGG orthology group: K01212, levanase [EC: 3.2.1.65] (inferred from 100% identity to bth:BT_1759)

Predicted SEED Role

"Levanase (EC 3.2.1.65)" (EC 3.2.1.65)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.65

Use Curated BLAST to search for 3.2.1.65

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A6W7 at UniProt or InterPro

Protein Sequence (610 amino acids)

>BT1759 levanase precursor (2,6-beta-D-fructofuranosidase) (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNVFLPVKQLQTFLICFILMTISVSTRAADSPLLIKNLGEGHCLVRVNTSQNYLLLPVED
ASPDVRISMIVNNKEVKNFDVRLAIHKVDYFVPVDLSDFSGKTVSFKFKMNSNDPIRVNL
SPDNTACCKEMKLSDTFDTTNREKFRPTYHFSPLYGWMNDPNGMVYKDGEYHLFYQYNPY
GSKWGNMNWGHAISKDLVNWEHRPVAIAPDALGTIFSGSAVVDHNNTAGFGAGAIIAIYT
QNSDRQVQSIAYSTDNGRTFTKYENNPVLVSEARDFRDPKVFWYEATKRWIMVLAVGQEM
QIFSSPNLKDWAFESSFGEGYGAHGNVWECPDLFELPVEGTNEKKWVLLCSLGDGPFGDS
ATQYFIGSFDGKKFSCDNQPNVTKWMDWGKDHYATVTWSDAPDNRRIAIAWMSNWQYAND
VPTSQYRSPNSIPRDLSLFAIDGGIYLQSAPSPELLKLRGVSKKRSFKVNGTRIVKDLIP
NNEGAYEIELSLKNQQAEIIGFRLYNDKGEEVDMQYDMKEKKFSMDRRKSGEVNFNENFP
MLTWTAIEEDKDEMKLRLFVDRSSVEAFGDGGRFAMTNQVFPSEPYNHISFYSKGGAYKV
DSFVVYKLKK