Protein Info for BT1615 in Bacteroides thetaiotaomicron VPI-5482

Annotation: aminoacyl-histidine dipeptidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01893: Xaa-His dipeptidase
  • amino acids 36 to 512 (477 residues), 601.2 bits, E=6.9e-185
• PF01546: Peptidase_M20
  • amino acids 101 to 509 (409 residues), 59.4 bits, E=4.8e-20
• PF07687: M20_dimer
  • amino acids 236 to 321 (86 residues), 47.3 bits, E=1.7e-16

Best Hits

Swiss-Prot: 49% identical to PEPD_ECOLI: Cytosol non-specific dipeptidase (pepD) from Escherichia coli (strain K12)

KEGG orthology group: K01270, aminoacylhistidine dipeptidase [EC: 3.4.13.3] (inferred from 100% identity to bth:BT_1615)

MetaCyc: 49% identical to peptidase D (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]; 3.4.13.18 [EC: 3.4.13.18]

Predicted SEED Role

"Aminoacyl-histidine dipeptidase (Peptidase D) (EC 3.4.13.3)" (EC 3.4.13.3)

MetaCyc Pathways

• glutathione degradation (DUG pathway) (1/2 steps found)
• muropeptide degradation (2/4 steps found)
• γ-glutamyl cycle (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Glutathione metabolism
• Histidine metabolism
• Urea cycle and metabolism of amino groups
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.4.13.3

Use Curated BLAST to search for 3.4.13.18 or 3.4.13.3

Sequence Analysis Tools

See Q8A7B0 at UniProt or InterPro

Protein Sequence (516 amino acids)

>BT1615 aminoacyl-histidine dipeptidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MSSDKSSKLTIFSNKRKQNEPPIPIIKEKSMEKKDLKPAGVFKYFEEICQVPRPSKKEEK
IIAYLKAFGAKHNLETNVDEAGNVLIKKPATPGKENFQTVILQSHIDMVCEKNNDVQHDF
LTDPIETEIDGEWLKAKGTTLGADNGIGVATELAILADDSIEHGPLECLFTVDEETGLTG
AFELKEGFMSGDILLNLDSEDEGEIFIGCAGGIDSVAEFSYKEVEVPAGYFFFKVEVKGL
KGGHSGGDIHLGRGNANKILNRFLSRMANRQDLYLCEINGGNLRNAIPREAYAICAVPED
AKHDVRTELNIFTSEVENELAVTEPDLKLVLESETPRKMAIDQDTTTRLLKALYAAPHGV
YAMSQDIPGLVETSTNLASVKMKPNHIIRIETSQRSSILSARNDMANTVRAVFQLAGADV
TFGEGYPGWKPNPHSAILEVAAESYKRLFGVEAKVKAIHAGLECGLFLDKYPTLDMISFG
PTLTGVHSPDERMHIPSVEKFWKHLLDILAHVPAKK