Protein Info for BT1577 in Bacteroides thetaiotaomicron VPI-5482
Annotation: pyridoxamine 5'-phosphate oxidase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PDXH_BACTN: Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
KEGG orthology group: K00275, pyridoxamine 5'-phosphate oxidase [EC: 1.4.3.5] (inferred from 100% identity to bth:BT_1577)MetaCyc: 47% identical to pyridoxine/pyridoxamine 5'-phosphate oxidase (Escherichia coli K-12 substr. MG1655)
Pyridoxal 5'-phosphate synthase. [EC: 1.4.3.5]; 1.4.3.5 [EC: 1.4.3.5]
Predicted SEED Role
"Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.4.3.5)
MetaCyc Pathways
- superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
- pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
- pyridoxal 5'-phosphate salvage I (5/6 steps found)
- pyridoxal 5'-phosphate salvage II (plants) (5/9 steps found)
- 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (4/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.4.3.5
Use Curated BLAST to search for 1.4.3.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8A7E7 at UniProt or InterPro
Protein Sequence (213 amino acids)
>BT1577 pyridoxamine 5'-phosphate oxidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482) MKNIADIRQEYTKSGLRESELPCDPLSLFSRWLQEAIDANVEEPTAIIVGTVSPEGRPST RTVLLKGLHDGKFIFYTNYESRKGRQLAQNPYISLSFVWHELERQVHIEGTAAKVSPEES DEYFRKRPYKSRIGARISPQSQPIASRMQLIRAFVKEAARWLGKEVERPDNWGGYAVTPT RMEFWQGRPNRLHDRFLYTLKTDGKWEINRLSP