Protein Info for BT1442 in Bacteroides thetaiotaomicron VPI-5482

Annotation: adenosylmethionine-8-amino-7-oxononanoate aminotransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR00433: biotin synthase" amino acids 91 to 380 (290 residues), 395.3 bits, see alignment E=1.7e-122 PF04055: Radical_SAM" amino acids 123 to 279 (157 residues), 69 bits, see alignment E=8.7e-23 PF06968: BATS" amino acids 291 to 381 (91 residues), 82.8 bits, see alignment E=2.5e-27 TIGR00508: adenosylmethionine-8-amino-7-oxononanoate transaminase" amino acids 392 to 801 (410 residues), 582.1 bits, see alignment E=5.1e-179 PF00202: Aminotran_3" amino acids 404 to 799 (396 residues), 410.4 bits, see alignment E=1.1e-126

Best Hits

Swiss-Prot: 100% identical to BIOAB_BACTN: Biotin biosynthesis bifunctional protein BioAB (bioB) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00833, adenosylmethionine-8-amino-7-oxononanoate aminotransferase [EC: 2.6.1.62] (inferred from 100% identity to bth:BT_1442)

Predicted SEED Role

"Biotin synthase (EC 2.8.1.6) / Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62, EC 2.8.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.6

Use Curated BLAST to search for 2.6.1.62 or 2.8.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A7T2 at UniProt or InterPro

Protein Sequence (804 amino acids)

>BT1442 adenosylmethionine-8-amino-7-oxononanoate aminotransferase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKKQRHIQTTRSLLSRFRYWGRKNYAAFASMGREFQIGHLHTNVVDVALRKQNAKVTIPY
HTFMTLQEIKDQVLAGFDISSAQATWLANMADSEALYAAAHEITITCASHEFDMCSIINA
KSGRCPENCKWCAQSSHYKTQAEIYDLLPAEECLRQAKYNESQDVNRFSLVTSGRKPSPK
QISQLCDAARLMRKHSSIQLCASLGLLNEEELRALHTAGITRYHCNLETAPSYFPTLCST
HTQEQKLATLDAARRVGMDICCGGIIGMGETMEQRIEFAFTLAELNVQSIPINLLSPIPG
TPLENEKALSEEEILRTIALFRFINPTAFLRFAGGRSQLTPEAMRKALFVGINSAIVGDL
LTTLGSKVSDDKKMILEEGYHFADSQFDREHLWHPYTSTTDPLPVYKVKRADGATITLED
GRTLIEGMSSWWCAVHGYNHPVLNQAAKDQLDKMSHVMFGGLTHDPAIELGKLLLPLVPP
SMQKIFYADSGSVAVEVALKMAVQYWYAAGKPDKNNFVTIRSGYHGDTWNAMSVCDPVTG
MHSLFGSSLPVRYFVPAPSSRFDGEWNPDEIIPLRETIEKHSKELAALILEPIVQGAGGM
WFYHPQYLREAEKLCKEHDILLIFDEIATGFGRTGKLFAWEHAGVEPDIMCIGKALTGGY
MTLSAVLASNQIADTISNHAPKAFMHGPTFMGNPLACAVACASVRLLLDSGWAENVKRIE
AQLKEELAPARKFPQVADVRILGAIGVIQTERSVSMAYMQRRFVEEGIWVRPFGKLVYLM
PPFIISPEQLSKLTSGVLKIVREM