Protein Info for BT1275 in Bacteroides thetaiotaomicron VPI-5482
Updated annotation (from data): L-fuculose kinase fucK (EC 2.7.1.51); D-ribulose kinase (EC 2.7.1.47)
Rationale: Specifically important for L-fucose utilization, along with fucose isomerase BT1273 and fuculose-phosphate aldolase BT1274. Also important for D-arabinose utilization, so it is probably a D-ribulokinase as well.
Original annotation: L-fuculose kinase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to RHAB_BACTN: Rhamnulokinase (rhaB) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
KEGG orthology group: K00879, L-fuculokinase [EC: 2.7.1.51] (inferred from 100% identity to bth:BT_1275)Predicted SEED Role
"L-fuculokinase (EC 2.7.1.51)" in subsystem L-fucose utilization (EC 2.7.1.51)
MetaCyc Pathways
- superpathway of fucose and rhamnose degradation (10/12 steps found)
- L-fucose degradation I (4/4 steps found)
- D-arabinose degradation I (2/2 steps found)
- D-arabinose degradation II (3/4 steps found)
- ribitol degradation I (1/2 steps found)
- D-arabinose degradation V (1/3 steps found)
- superpathway of pentose and pentitol degradation (12/42 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.51
Use Curated BLAST to search for 2.7.1.47 or 2.7.1.51
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8JZS7 at UniProt or InterPro
Protein Sequence (476 amino acids)
>BT1275 L-fuculose kinase fucK (EC 2.7.1.51); D-ribulose kinase (EC 2.7.1.47) (Bacteroides thetaiotaomicron VPI-5482) MKDTTRNTYLAVDFGGGSGRVIAGSLLQGKLELEEIHRFTNRQVKLGNHVYWDFPALFED MKTGLKLAAQKGYHVKGIGIDTWGVDFGLIDKKGNLLGNPVCYRDARTDGMPDKVFQILD AQKHYACTGIQVMPINTLFQLYSMQQNQDVLLEVAQRLLFMPDLFSYYLTGVANNEYCIA STSELLDARQRNWSMDTIRALGLPEHLFGEIILPGTVRGTLKEEIGRETGLGPVDIIAVG SHDTASAVAAVPATEGQVAFLSSGTWSLLGVEVDEPILTEEARLAQFTNEGGVGGHIRFL QNITGLWILQRLMSEWKLRGEEQSYDTILPQAADAEIDTIIPVDDAEFMNPENMETALLN YCRNHSLKVPGNKAEMVKCVLQSLAFKYREAVAQLNRCLPSPIHRLNIIGGGSQNKLLNQ LTANALGIPVYAGPVEATAMGNILTQAMAKGEISSLREIREVVSHSVTPQVYYPEK