Protein Info for BT1155 in Bacteroides thetaiotaomicron VPI-5482

Annotation: Na+-translocating NADH-quinone reductase subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01941: NADH:ubiquinone oxidoreductase, Na(+)-translocating, F subunit" amino acids 5 to 422 (418 residues), 572.2 bits, see alignment E=3e-176 PF00111: Fer2" amino acids 38 to 114 (77 residues), 37.7 bits, see alignment E=2.5e-13 PF00970: FAD_binding_6" amino acids 205 to 276 (72 residues), 26.8 bits, see alignment E=8.3e-10 PF00175: NAD_binding_1" amino acids 289 to 403 (115 residues), 43.9 bits, see alignment E=5e-15

Best Hits

Swiss-Prot: 53% identical to NQRF_ACTSZ: Na(+)-translocating NADH-quinone reductase subunit F (nqrF) from Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / 130Z)

KEGG orthology group: K00351, Na+-transporting NADH:ubiquinone oxidoreductase subunit F [EC: 1.6.5.-] (inferred from 100% identity to bth:BT_1155)

MetaCyc: 50% identical to Na(+)-translocating NADH-quinone reductase subunit F (Vibrio cholerae)
TRANS-RXN-214 [EC: 7.2.1.1]

Predicted SEED Role

"Na(+)-translocating NADH-quinone reductase subunit F (EC 1.6.5.-)" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes (EC 1.6.5.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.-

Use Curated BLAST to search for 1.6.5.- or 7.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A8L2 at UniProt or InterPro

Protein Sequence (422 amino acids)

>BT1155 Na+-translocating NADH-quinone reductase subunit (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MNLILSSIGVFLVVILLLVVILLVAKKFLVPSGNVKLTINGETGLEVESGSTLLNTLAVN
GVFLSSACGGKGSCGQCKCQVLEGGGEILPSEVPHFSRKQQQDHWRLGCQVKVKSDMAIK
IDESVLGVKEWECEVISNKNVATFIKEFIVALPKGEHMDFIPGSYAQIKIPKFSMDYDKD
IDKSLIGEEYLPAWEKFGLLGLKCKNEEETIRAYSMANYPAEGDRIMLTVRIATPPFKPK
EQGPGFMDVMPGIASSYIFTLKPGDKVIMSGPYGDFHPIFDSNKEMMWIGGGAGMAPLRA
QIMHLTKTLHTTDRKMSYFYGARALNEVFYLEDFLQIEKDFPNFTFHLALDRPDPAADAA
GVKYTPGFVHNVIYETYLKNHEAPEDIEYYMCGPGPMSKAVEKMLDDLGVPAQNLMFDNF
GG