Protein Info for BT0899 in Bacteroides thetaiotaomicron VPI-5482

Annotation: DNA gyrase subunit A (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 858 TIGR01063: DNA gyrase, A subunit" amino acids 7 to 812 (806 residues), 1156.8 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 31 to 469 (439 residues), 589.4 bits, see alignment E=4.6e-181 PF03989: DNA_gyraseA_C" amino acids 502 to 546 (45 residues), 36.4 bits, see alignment 2.9e-13 amino acids 553 to 596 (44 residues), 30.8 bits, see alignment 1.6e-11 amino acids 612 to 656 (45 residues), 32.4 bits, see alignment 5e-12 amino acids 661 to 709 (49 residues), 44.4 bits, see alignment 9.1e-16 amino acids 715 to 761 (47 residues), 48.6 bits, see alignment 4.4e-17 amino acids 766 to 806 (41 residues), 37.1 bits, see alignment 1.7e-13

Best Hits

Swiss-Prot: 54% identical to GYRA_BACHD: DNA gyrase subunit A (gyrA) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 100% identity to bth:BT_0899)

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A9B6 at UniProt or InterPro

Protein Sequence (858 amino acids)

>BT0899 DNA gyrase subunit A (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MLEQDRIIKINIEEEMKSSYIDYSMSVIVSRALPDVRDGFKPVHRRILYGMMELGNTSDK
PYKKSARIVGEVLGKYHPHGDSSVYYAMVRMAQEWAMRYPLVDGQGNFGSVDGDSPAAMR
YTEARLNKLGEAMMDDLYKETVDFEPNFDNTLTEPKVMPTRIPNLLVNGASGIAVGMATN
MPPHNLSEVIDACEAYIDNQEITVEELMTYVKAPDFPTGGYIYGISGVREGYLTGRGRVV
MRAKAEIETGQTHDKIVVTEIPYNVNKAELIKYIADLVNDKRIEGISNANDESDRDGMRI
VIDVKRDANASVVLNKLYKMTALQTSFGVNNVALVHGRPKTLNLRDMIKYFVEHRHEVVI
RRTQFDLRKAKERAHILEGLIIASDNIDEVIRIIRAAKTPNDAIAGLIERFNLTEIQSRA
IVEMRLRQLTGLMQDQLHAEYEEIMKQIAYLESILADDEVCRKVMKDELLEVKAKYGDER
RSEIVYSSEEFNPEDFYADDQMIITISHMGYIKRTPLTEFRAQNRGGVGSKGTETRDADF
VEHIYPATMHNTMMFFTQKGKCYWLKVYEIPEGTKNSKGRAIQNLLNIDSDDSVTAYLRV
KSLDDTEYINSHYVLFCTKKGVIKKTLLEQYSRPRQNGVNAITIREDDSVIEVRMTNGNN
EIIIANRNGRAIRFHEAAVRVMGRTATGVRGITLDNDGQDEVIGMICIKDLETESVMVVS
EQGYGKRSEIEDYRKTNRGGKGVKTMNITEKTGKLVTIKSVTDENDLMIINKSGITIRLK
VADVRIMGRATQGVRLINLEKRNDQIGSVCKVMTESLEDEVPAEEAEGTIVSDLTTDQDV
DNADTATDVNENNNEIEE