Protein Info for BT0620 in Bacteroides thetaiotaomicron VPI-5482

Annotation: Na+-transporting NADH:ubiquinone oxidoreductase, Electron transport complex protein rnfG (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 227 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR01947: electron transport complex, RnfABCDGE type, G subunit" amino acids 3 to 196 (194 residues), 137.2 bits, see alignment E=3.4e-44 PF04205: FMN_bind" amino acids 91 to 193 (103 residues), 69 bits, see alignment E=2.3e-23

Best Hits

KEGG orthology group: K03612, electron transport complex protein RnfG (inferred from 100% identity to bth:BT_0620)

Predicted SEED Role

"Electron transport complex protein RnfG" in subsystem Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8AA45 at UniProt or InterPro

Protein Sequence (227 amino acids)

>BT0620 Na+-transporting NADH:ubiquinone oxidoreductase, Electron transport complex protein rnfG (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MLLVLTGVTAVSVALLAYVNELTKGPIAEANAKTLNEALKKVLPEYTNNPVAESDTVFSE
KDGKKIVDFIIYPAKNGEKWVGSAVEAKSMGFGGELKVLVGFDAEGKIYNYSLLAHAETP
GLGSKADKWFGAYDPAKNEKPVSHEESAKSILGMNPGETPLAVTKDMNGQVDAITASTIT
SRAFLNAVNAAYQAYKGGEVDTNSGASQKNEPAVAPEGEAAANDTNN