Protein Info for BT0571 in Bacteroides thetaiotaomicron VPI-5482
Annotation: phenylacetate-coenzyme A ligase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to PAAK_THET2: Phenylacetate-coenzyme A ligase (TT_C0602) from Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to bth:BT_0571)Predicted SEED Role
"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)
MetaCyc Pathways
- penicillin G and penicillin V biosynthesis (1/4 steps found)
- phenylacetate degradation II (anaerobic) (1/4 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (2/9 steps found)
- phenylacetate degradation I (aerobic) (1/9 steps found)
- superpathway of phenylethylamine degradation (1/11 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (2/27 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.30
Use Curated BLAST to search for 6.2.1.30
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8AA94 at UniProt or InterPro
Protein Sequence (432 amino acids)
>BT0571 phenylacetate-coenzyme A ligase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482) MIWNETIECMDRESLRKIQSIRLKKIVDYVYHNTPFYRKKMQEMGITPDDINTIDDIVKL PFTTKHDLRDNYPFGLCAVPMSQIVRIHASSGTTGKPTVVGYTRKDLSSWAECISRAFTA YGAGRSDIFQVSYGYGLFTGGLGAHAGAENIGASVIPMSSGNTEKQITLMHDFGSTVLCC TPSYALYLADAIKDSGYPREEFQLKVGALGAEPWTENMRHEIEEKLGIKAYDIYGLSEIA GPGVGYECECQHGTHLNEDHYFPEIIDPNTLQPVEPGQTGELVFTHLTKEGMPLLRYRTR DLTALHYDKCSCGRTLVRMDRILGRSDDMLIIRGVNVFPTQIESVILEMEEFEPHYLLIV GRENNTDTMELQVEVRPDFYSDEINRMLALKKKLASRLQSVLGLGVNVKLVEPRSIERSV GKAKRVIDNRKI