Protein Info for BT0557 in Bacteroides thetaiotaomicron VPI-5482

Annotation: carbamyl phosphate synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1057 (1056 residues), 1577 bits, E=0
• PF25596: CPSase_L_D1
  • amino acids 5 to 122 (118 residues), 150.3 bits, E=1.1e-47
  • amino acids 561 to 672 (112 residues), 151.7 bits, E=3.9e-48
• PF02786: CPSase_L_D2
  • amino acids 126 to 327 (202 residues), 271.7 bits, E=2e-84
  • amino acids 676 to 876 (201 residues), 94.7 bits, E=3e-30
• PF02222: ATP-grasp
  • amino acids 133 to 279 (147 residues), 32.6 bits, E=3.2e-11
  • amino acids 683 to 847 (165 residues), 47.5 bits, E=8.6e-16
• PF07478: Dala_Dala_lig_C
  • amino acids 141 to 297 (157 residues), 41.6 bits, E=5.2e-14
  • amino acids 694 to 845 (152 residues), 32.2 bits, E=4e-11
• PF02787: CPSase_L_D3
  • amino acids 417 to 499 (83 residues), 97.7 bits, 1.6e-31
• PF08443: RimK
  • amino acids 674 to 778 (105 residues), 24.2 bits, E=1.2e-08
• PF02142: MGS
  • amino acids 958 to 1046 (89 residues), 82.3 bits, E=1.1e-26

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (16/18 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (9/9 steps found)
• UMP biosynthesis II (6/6 steps found)
• UMP biosynthesis III (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-arginine biosynthesis II (acetyl cycle) (8/10 steps found)
• UMP biosynthesis I (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline degradation (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (2/4 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (1/4 steps found)
• superpathway of arginine and polyamine biosynthesis (10/17 steps found)
• L-arginine biosynthesis IV (archaea) (3/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (3/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See Q8AAA8 at UniProt or InterPro

Protein Sequence (1075 amino acids)

>BT0557 carbamyl phosphate synthetase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKENIKKVLLLGSGALKIGEAGEFDYSGSQALKALKEEGIETILINPNIATVQTSEGVAD
QIYFLPVTPYFVEKVIQKEKPEGIMLAFGGQTALNCGVALYKEGILEKYNVKVLGTPVQA
IMDTEDRELFVQKLNEINVKTIKSEAVENAEDARRAAKELGYPVIVRAAYALGGLGSGFC
DNEEQLDVLVEKAFSFSPQVLVEKSLRGWKEVEYEVVRDRFDNCITVCNMENFDPLGIHT
GESIVIAPSQTLTNSEYHKLRELAIRIIRHIGIVGECNVQYAFDPESEDYRVIEVNARLS
RSSALASKATGYPLAFVAAKLGLGYGLFDLKNSVTKTTSAFFEPALDYVVCKIPRWDLGK
FHGVDKELGSSMKSVGEVMAIGRTFEEAIQKGLRMIGQGMHGFVENKELVIPDIDKALRE
PTDKRIFVISKAFRAGYTIDQVHELTKIDKWFLQKLMNIMKTSEEMHEWGNNHKQIADLP
VELLRKAKVQGFSDFQIARAIGYEGDMENGSLYVRKYRKAAGILPVVKQIDTLAAEYPAQ
TNYLYLTYSGVANDVHYLGDHKSIVVLGSGAYRIGSSVEFDWCGVQALNTIRKEGWRSVM
INYNPETVSTDYDMCDRLYFDELTFERVMDILELENPHGVIVSTGGQIPNNLALRLDAQN
IHILGTSAQSIDNAEDREKFSAMLDRIGVDQPRWRELTSLEDINEFVDEVGFPVLVRPSY
VLSGAAMNVCSNQEELERFLKLAANVSKKHPVVVSQFIEHAKEVEMDAVAQNGEIIAYAI
SEHIEFAGVHSGDATIQFPPQKLYVETVRRIKRISREIAKALNISGPFNIQYLAKDNDIK
VIECNLRASRSFPFVSKVLKINFIELATKVMLGLPVEKPEKNLFELDYVGIKASQFSFNR
LQKADPVLGVDMASTGEVGCIGSDTSCAVLKAMLSVGYRIPKKNILLSTGTMKQKADMMD
AARMLVNKGYKLFATGGTHKTFAENGIESTLVYWPSEEGHPQALEMLHNKEIDMVVNIPK
NLTAGELDNGYKIRRAAIDLNVPLITNARLASAFINAFCTMTVDDIAIKSWEEYK