Protein Info for BT0535 in Bacteroides thetaiotaomicron VPI-5482

Annotation: iron-containing alcohol dehydrogenase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00465: Fe-ADH
  • amino acids 9 to 348 (340 residues), 281.9 bits, E=6.8e-88
• PF13685: Fe-ADH_2
  • amino acids 16 to 106 (91 residues), 29.8 bits, E=5.2e-11
  • amino acids 147 to 278 (132 residues), 29.9 bits, E=4.9e-11

Best Hits

Swiss-Prot: 50% identical to YQHD_ECOLI: Alcohol dehydrogenase YqhD (yqhD) from Escherichia coli (strain K12)

KEGG orthology group: K08325, NADP-dependent alcohol dehydrogenase [EC: 1.1.-.-] (inferred from 100% identity to bth:BT_0535)

MetaCyc: 50% identical to NADPH-dependent aldehyde reductase YqhD (Escherichia coli K-12 substr. MG1655)
Aldehyde reductase. [EC: 1.1.1.21]; Alcohol dehydrogenase (NADP(+)). [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.2 [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.2 [EC: 1.1.1.21, 1.1.1.2]; 1.1.1.- [EC: 1.1.1.21, 1.1.1.2]

Predicted SEED Role

"Hypothetical oxidoreductase YqhD (EC 1.1.-.-)" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.-.-)

MetaCyc Pathways

• methylglyoxal degradation III (1/2 steps found)
• 1,3-propanediol biosynthesis (engineered) (6/9 steps found)
• D-arabinose degradation V (1/3 steps found)
• D-xylose degradation to ethylene glycol (engineered) (1/4 steps found)
• superpathway of methylglyoxal degradation (3/8 steps found)
• glycerol degradation to butanol (7/16 steps found)
• pyruvate fermentation to butanol I (1/8 steps found)
• detoxification of reactive carbonyls in chloroplasts (1/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (2/13 steps found)
• superpathway of pentose and pentitol degradation (12/42 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Bisphenol A degradation
• Caprolactam degradation
• Ethylbenzene degradation
• Fructose and mannose metabolism
• Galactose metabolism
• Glycerolipid metabolism
• Glycolysis / Gluconeogenesis
• Limonene and pinene degradation
• Naphthalene and anthracene degradation
• Pentose and glucuronate interconversions
• Pyruvate metabolism
• Retinol metabolism
• gamma-Hexachlorocyclohexane degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.-.- or 1.1.1.2 or 1.1.1.21

Sequence Analysis Tools

See Q8AAD0 at UniProt or InterPro

Protein Sequence (381 amino acids)

>BT0535 iron-containing alcohol dehydrogenase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MENFIFQNPVKLIMGHGMIARLSKEIPSDKRIMITFGGGSVKKNGVYDQVKEALKDHFTI
EFWGIEPNPAIETLRKAIALGKEQKVDYLLAVGGGSVIDGTKLISAGLLYDGDAWDLVLA
GRPVTKTVPLSTVLTLPATGSEMNNGAVISRHETKEKYPFYSNFPLFSILDPEVTFTLPP
HQVACGLADTFVHVMEQYMTVAGQSRVMDRWAEGILQTLVEIAPKIRENQHDYQLMADFM
LSATMALNGFIAMGVSQDWATHMIGHEITALHGLTHGHTLVIILPATLRVLREAKGDKLV
QYGERVWGITSGTKEERIDEAIDRTEEFFRSLGLTTRLHEENIGEETVLEIERRFNERGA
KYGENENVTGAVARKILEAAL