Protein Info for BT0458 in Bacteroides thetaiotaomicron VPI-5482

Annotation: beta-mannosidase precursor (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 864 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00703: Glyco_hydro_2" amino acids 222 to 331 (110 residues), 40.7 bits, see alignment E=5.5e-14 PF17786: Mannosidase_ig" amino acids 689 to 781 (93 residues), 67.9 bits, see alignment E=1.3e-22 PF17753: Ig_mannosidase" amino acids 784 to 855 (72 residues), 48.3 bits, see alignment E=9.9e-17

Best Hits

KEGG orthology group: K01192, beta-mannosidase [EC: 3.2.1.25] (inferred from 100% identity to bth:BT_0458)

Predicted SEED Role

"Beta-mannosidase (EC 3.2.1.25)" in subsystem Mannose Metabolism (EC 3.2.1.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.25

Use Curated BLAST to search for 3.2.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8AAK6 at UniProt or InterPro

Protein Sequence (864 amino acids)

>BT0458 beta-mannosidase precursor (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MILNLTGKIAPIACGLLCCCSMVYAQGNDTSEVMLLDTGWEFSQSGTEKWMPATVPGTVH
QDLISHELLPNPFYGMNEKKIQWVENEDWEYRTSFIVSEEQLNRDGIQLIFEGLDTYADV
YLNGSLLLKADNMFVGYTLPVKSVLRKGENHLYIYFHSPIRQTLPQYASNGFNYPADNDH
HEKHLSVFSRKAPYSYGWDWGIRMVTSGVWRPVTLRFYDIATISDYYVRQLSLTDENARL
SNELIVNQIVPQKIPAEVRVNVSLNGTTVTEVKQQVTLQPGINHITLPAEVTNPVRWMPN
GWGTPTLYDFSAQIACGDRIVAEQSHRIGLRTIRVVNEKDKDGESFYFEVNGIPMFAKGA
NYIPQDALLPNVTTERYQTLFRDMKEANMNMVRIWGGGTYENNLFYDLADENGILVWQDF
MFACTPYPSDPTFLKRVEAEAVYNIRRLRNHASLAMWCGNNEILEALKYWGFEKKFTPEV
YQGLMHGYDKLFRELLPSTVKEFDSDRFYVHSSPYLANWGRPESWGTGDSHNWGVWYGKK
PFESLDTDLPRFMSEFGFQSFPEMKTIAAFAAPEDYQIESEVMNAHQKSSIGNSLIRTYM
ERDYIIPESFEDFVYVGLVLQGQGMRHGLEAHRRNRPYCMGTLYWQLNDSWPVVSWSSID
YYGNWKALHYQAKRAFAPVLINPIQQNDSLSVYLISDRLDTMEQMTLEMKVVDFDGKTLG
KKIQVHSLEVPANTSKCVYRAKLDGWLTPEDCRRSFLKLILKDKSGHQVAESVHFFRKTK
DLQLPPTSVSYQMKQTDGKCELTLFSSMLAKDIFIETPLQGARYSDNFFDLLPGERKKVI
ITSPRIKKGEELPVNIKHIRETYK