Protein Info for BT0355 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): L-arabinose/L-arabinobiose symporter
Rationale: Specifically important for L-arabinose utilization and also required for arabinobiose utilization (see PMC5061871). PMC5061871 proposed that it takes up arabinobiose, but because the arabinobiose hydrolase was not identified, it is not certain if arabinobiose is transported. Also important for D-arabinose utilization, but this seems to be a polar effect.
Original annotation: Na+/glucose cotransporter (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 43 to 66 (24 residues), see Phobius details amino acids 79 to 96 (18 residues), see Phobius details amino acids 117 to 140 (24 residues), see Phobius details amino acids 155 to 175 (21 residues), see Phobius details amino acids 182 to 204 (23 residues), see Phobius details amino acids 241 to 259 (19 residues), see Phobius details amino acids 281 to 305 (25 residues), see Phobius details amino acids 346 to 375 (30 residues), see Phobius details amino acids 388 to 409 (22 residues), see Phobius details amino acids 424 to 442 (19 residues), see Phobius details amino acids 451 to 471 (21 residues), see Phobius details amino acids 491 to 512 (22 residues), see Phobius details amino acids 544 to 563 (20 residues), see Phobius details PF00474: SSF" amino acids 34 to 457 (424 residues), 294.3 bits, see alignment E=7.6e-92 TIGR00813: transporter, solute:sodium symporter (SSS) family" amino acids 34 to 457 (424 residues), 353.7 bits, see alignment E=7.8e-110

Best Hits

KEGG orthology group: K03307, solute:Na+ symporter, SSS family (inferred from 100% identity to bth:BT_0355)

Predicted SEED Role

"Predicted sodium-dependent galactose transporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8AAV7 at UniProt or InterPro

Protein Sequence (564 amino acids)

>BT0355 L-arabinose/L-arabinobiose symporter (Bacteroides thetaiotaomicron VPI-5482)
MEALDWLVIGVFFLALIGIIVWVVRQKQNDSADYFLGGRDATWLAIGASIFASNIGSEHL
IGLAGAGASSGMAMAHWEIQGWMILILGWVFVPFYSRSMVYTMPEFLERRYNPQSRTILS
VISLVSYVLTKVAVTVYAGGLVFQQVFGIKELWGIDFFWIAAIGLVVLTALYTIFGGMKS
VLYTSVLQTPILLLGSLIILVLGFKELGGWDEMMRVCGAVTVNDYGDTMTNLIRSNDDAN
FPWLGALIGSAIIGFWYWCTDQFIVQRVLSGKNEKEARRGTIFGAYLKLLPVFLFLIPGM
IAFALHQKYIGAGGEGFLPMLANGTANADAAFPTLVAKLLPAGVKGLVVCGILAALMSSL
ASLFNSSAMLFTIDFYKRFRPETPEKKLVGIGQIATVVIVILGILWIPIMRSVGDVLYTY
LQDVQSVLAPGIAAAFLLGICWKRTSAQGGMWGLIAGMIIGLTRLGAKVYYSNAGEVADS
TFKYLFYDMNWLFFCGWMFLFCIIVVIVVSLATEAPTAEKIQGLVFGTATKEQKAATRAS
WDHWDIIHTVIILAITGAFYWYFW