Protein Info for BT0309 in Bacteroides thetaiotaomicron VPI-5482
Annotation: Dihydrolipoamide dehydrogenase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00382, dihydrolipoamide dehydrogenase [EC: 1.8.1.4] (inferred from 100% identity to bth:BT_0309)Predicted SEED Role
"Dihydrolipoamide dehydrogenase of acetoin dehydrogenase (EC 1.8.1.4)" in subsystem Acetoin, butanediol metabolism (EC 1.8.1.4)
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- glycine biosynthesis II (3/3 steps found)
- glycine cleavage (3/3 steps found)
- 2-oxoglutarate decarboxylation to succinyl-CoA (2/3 steps found)
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (2/3 steps found)
- pyruvate decarboxylation to acetyl CoA I (2/3 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Citrate cycle (TCA cycle)
- Glycine, serine and threonine metabolism
- Glycolysis / Gluconeogenesis
- Pyruvate metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.4
Use Curated BLAST to search for 1.8.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8AB03 at UniProt or InterPro
Protein Sequence (447 amino acids)
>BT0309 Dihydrolipoamide dehydrogenase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482) MNFDIAIIGGGPAGYTAAERAGANGLRAVLFEKKAIGGVCLNEGCIPTKTLLYSAKILDS IKSASKYGISAESPSFDLTKIMSRKEKTVKMLTGGVKMTVNSYGVTIVEKEAFIEGEENG LIRIICDGERYEVKYLLVCTGSDTVIPPIPGLSEVSYWTSKEALEIKELPETLVVIGGGV IGMEFASFFNSMGVKVHVVEMMPEILGVMDKETSSMLRMEYAKRGVTFYLNTKVIEVKPD GVVIEKDGKASTIKTEKILLSVGRKANITNVGLDKLNIELHRNGVKVDEYLQTSHPGVYA CGDITGYSLLAHTAIREAEVAINHILGVEDRMNYNSVPGVVYTNPEVAGVGKTEEELTKQ GIPYRVTKLPMAYSGRFVAENEQVNGICKLILDEADHIIGCHMLGNPASELIVIAGIAIQ KGYTVEEFQKNVFPHPTVGEIYHEVLS